Spinning Disk Confocal Microscope with FRAP and photo-activation
Nikon Inverted Spinning Disk Confocal Microscope
The inverted Nikon spinning disk confocal microscope is equipped with four laser lines (405, 488, 561, and 647 nm), 4x-40x air objective lenses, and 60x NA1.40 and 100x NA1.45 oil objective lenses. Dual EM-CCD cameras for widefield (Hamamatsu ImageEM) and confocal imaging (Andor 897) are used. A separate high-intensity 405 nm laser can be used for photobleaching, photoconversion, and photoactivation imaging. A Tokai-Hit incubation chamber with single 35mm dish or a multi-well chamber slide can be used on this microscope for live imaging. This microscope can be used for a wide range of applications and is particularly well suited to fast image acquisition of dim samples, live imaging, and large area scanning.
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