Instrument/Service Type:
Widefield microscope
Make/Model:
IX81
Instrument Description:
This microscope uses a high-intensity Prior Scientific Lumen 200Pro 200-watt metal halide lamp, motorized filter wheels with Chroma and Semrock optical filters, a Prior Scientific H117 motorized, linear-encoded stage (X-Y), and Olympus motorized Z-drive. The optical filters include QD360, DAPI/Hoechst, ECFP, EGFP, Venus, Alexa568;TxRed;mCherry/TdTomato, Alexa647, and other related probes. The microscope allows custom configurations. There is also brightfield and phase-contrast (20x and 40x). The detector is a Hamamatsu ORCA R2 CCD.
The following objective lenses are available: 4x NA 0.13, 10x NA 0.40, 20x NA 0.70, 40x NA 0.75, 60x water NA 1.20, 60x oil NA 1.35, and 100x oil NA 1.40.
For live cell imaging, an InVivo Scientific stage-top incubation chamber is available in single 35 mm dishes or multiwell plate formats, using pre-blended atmospheric gas. The microscope is controlled with Slidebook 6.0 software and is excellent for fixed samples and timelapse imaging of live cells.
Instrument Website URL:
https://www.colorado.edu/lmcf/microscopes-workstations/widefield-stereo
Primary Contact:
James Orth
Email: james.orth@colorado.edu
Phone: 303-492-5955 or 303-492-5057
Instrument Home Department/Institute:
MCDB
Instrument Home Facility:
Light Microscopy Core Facility
Instrument Location:
Porter B047A
Available to university users?
Yes
Available to external, academic and national lab users?
Yes