The use of animals in research, testing and education is subject to a multitude of laws, policies, regulations and standards. Two such laws/policies include the Animal Welfare Act (AWA) and the Public Health Service (PHS) policy, which require documentation that personnel are appropriately trained in animal care and use.  All personnel conducting research with vertebrate animals must be appropriately qualified and trained before working with animals.

The principal investigator (PI) is responsible for providing documentation that all personnel listed in a protocol have completed the required animal care and use training, and that the certificates of completion are included with the protocol when submitted (or that the certificates of completion are on file in the IACUC office).

Everyone named on the protocol—including students, lab techs, and visiting scholars—are required to complete the online CITI courses, available under the sections Investigators, Staff and Students, Post-Approval Monitoring (PAM), Reducing Pain and Distress in Laboratory Mice and Rats, Post-Procedure Care of Mice & Rats in Research: Minimizing Pain & Distress, and other CITI modules appropriate for the planned work. For example, if the protocol proposes the use of mice or rats, all named personnel must complete Working with Mice in Research and Working with Rats in Research Settings. If working with zebrafish or other species you must also complete species-specific training courses. Personnel working with wildlife must also complete the Wildlife Research section.

Procedure-specific hands-on training may be obtained through the individual investigator’s laboratory or by contacting the Office of Animal Research (OAR) via

NOTE: All principal investigators with protocols and key personnel handling IACUC submissions or training should be on the listserv. If you need to be added to the IACUC listserv, please contact

Mandatory Training

There are four requirements to complete before working with animals at the University of Colorado at Boulder:

  1. CITI Collaborative Institutional Training Initiative
    An online IACUC basic training.
    ** Once you have completed and passed the CITI courses, please print your certificate of completion and send to iacuc@colorado.eduCompletion of CITI modules is verified by the IACUC Office at the time of protocol application, continuing review, and when new personnel (including students) are added to the protocol.
    ** Note: You may take the IACUC CITI Training Courses prior to or concurrently with the Occupational Health & Safety Training.
  2. Occupational Health & Safety Training (see schedule below)
    The Office of Environmental Health & Safety administers the Occupational Health Program for CU Boulder employees and certain students who may be exposed to health risks as part of their jobs or research activities at CU Boulder. You must sign up in advance to enroll for this training. For enrollment, go to

3. Your principal investigator (PI) must submit an addendum via TOPAZ to add you to their approved protocol. 
NOTE: Once you have completed the Occupational Health & Safety Training, EH&S will submit your information to eRA. At this time, your Principal Investigator will be able to add you to their protocol.

4. Submit a Facility Access Request.
NOTE: You must have completed the IACUC CITI Training and Occupational Health & Safety Training before you can request facility access.

Supplemental Training (optional)

These are voluntary additional training modules for personnel involved in research with vertebrate animals who wish to further their training:

Training for IACUC Members

Prior to performing IACUC duties or voting on proposals submitted to the IACUC, members must contact the IACUC Office to acquire training and attend two convened IACUC meetings. A general introductory/training meeting with the IACUC Director will be required prior to performing IACUC duties.

Once IACUC members have completed the mandatory training, they may be paired with experienced IACUC members who will serve as their mentor.

How to Access the IACUC CITI Online Training Modules

  • Go to On the home page, click Create an Account link in the right-hand corner of the screen.
  • The next screen will ask you for the Organization Affiliation. Type in University of Colorado at Boulder and check off the “I Agree to the Terms of Service” confirmation. Continue to step 2.
  • Enter your personal details and click the Continue to Step 3 button to continue.
  • Enter your username and password (this will be your email address and IdentiKey).
  • Complete the security question and answer boxes then click Continue to Step 4.
  • Input your “Country of Residence” then click on Continue to Step 5.
  • Answer all applicable questions then click on Continue to Step 6.
  • Continue through all steps.
  • Once you have created your account, you will then have access to the CITI modules for the University of Colorado Boulder. If you do not see the course you need, select Add a Course to add it.
  • Questions? Contact the IACUC Director at (303) 492-4787 or

Please note:

a. After you have created your account, future access can be obtained by selecting Log in via SSO on your home page.

b. In the future, if you need to be provided with your username and password, please use the “forgot username or password” link, which is adjacent to the login area on the homepage. This utility emails you your username and password.