The use of animals in research, testing and education is subject to a multitude of laws, policies, regulations and standards. Two such laws/policies include the Animal Welfare Act (AWA) and the Public Health Service (PHS) policy, which require documentation that personnel are appropriately trained in animal care and use.  All personnel conducting research with vertebrate animals must be appropriately qualified and trained before working with animals.

The principal investigator (PI) is responsible for providing documentation that all personnel listed in a protocol have completed the required animal care and use training, and that the certificates of completion are included with the protocol when submitted (or that the certificates of completion are on file in the IACUC office).

Everyone named on the protocol—including students, lab techs, and visiting scholars—are required to complete the online CITI courses, available under the sections Investigators, Staff and Students, Post-Approval Monitoring (PAM), Reducing Pain and Distress in Laboratory Mice and Rats, Post-Procedure Care of Mice & Rats in Research: Minimizing Pain & Distress, and other CITI modules appropriate for the planned work. For example, if the protocol proposes the use of mice or rats, all named personnel must complete Working with Mice in Research and Working with Rats in Research Settings. If working with zebrafish or other species you must also complete species-specific training courses. Personnel working with wildlife must also complete the Wildlife Research section.

Procedure-specific hands-on training may be obtained through the individual investigator’s laboratory or the Office of Animal Research via the OAR Training Request Form.

NOTE: All principal investigators with protocols and key personnel handling IACUC submissions or training should be on the listserv. If you need to be added to the IACUC listserv, please contact

Mandatory Training

There are four requirements to complete before working with animals at the University of Colorado at Boulder:

  1. IACUC CITI Training
    • Select the Log in through my institution option so that your training is associated with your CU Boulder profile. (If this is your first time using CITI at CU Boulder, you will be asked to set up a profile.)
    • You may take the IACUC CITI Training Courses prior to or concurrently with the Occupational Health & Safety Training.
    • Questions? Contact the IACUC Director at (303) 492-4787 or
  2. Occupational Health & Safety Training
    • The Office of Environmental Health & Safety (EH&S) administers the Occupational Health Program for employees and students who may be exposed to health risks as part of their jobs or research activities at CU Boulder. You must sign up in advance to enroll for this training.
  3. Your principal investigator (PI) must submit an addendum via TOPAZ to add you to their approved protocol
    • Your Principal Investigator will not be able to add you to their protocol until you have completed Step 2: Occupational Health & Safety Training.
  4. Facility Access Request Form
    • Please complete both Step 1: IACUC CITI Training and Step 2: Occupational Health & Safety Training before submitting a Facility Access Request.

Supplemental Training (optional)

These are voluntary additional training modules for personnel involved in research with vertebrate animals who wish to further their training:

Training for IACUC Members

Prior to performing IACUC duties or voting on proposals submitted to the IACUC, members must contact the IACUC office to acquire training and attend two convened IACUC meetings. A general introductory/training meeting with the IACUC Director will be required prior to performing IACUC duties.

Once IACUC members have completed the mandatory training, they may be paired with experienced IACUC members who will serve as their mentor.