Theodore W. Randolph

Theodore RandolphGillespie Professor
Center for Pharmaceutical Biotechnology, Co-Director
JSCBB C227
(303) 492-4776
theodore.randolph@colorado.edu
Curriculum Vitae

Education

B.S., University of Colorado (1983)
Ph.D. University of California, Berkeley (1987)

Awards

  • College of Engineering Dean’s Faculty Fellowship 2011-12
  • AAPS Dale E. Wurster Research Award in Pharmaceutics, 2010
  • Triennial John M. Prausnitz Award in Applied Chemical Thermodynamics, 2009
  • Bioscience Company of the Year - BaroFold, Inc., co-founded by Ted Randolph
  • American Society of Engineering Educators Dow Lectureship Award, 2007
  • Editorial Board Member J. Pharmaceutical Innovation
  • Ebert Award, Best Original Investigation in 2006, American Pharmacists Association
  • American Institute of Chemical Engineers, Professional Progress Award, 2005
  • Boulder Faculty Assembly Research and Creative Work Award 2003
  • College of Engineering and Applied Sciences Max Peters Award for Outstanding Service 2002
  • Editorial Board Member, Journal of Pharmaceutical Sciences
  • Outstanding Graduate Teaching Award, Department of Chemical Engineering, 2000
  • Editorial Board Member, Current Pharmaceutical Biotechnology
  • College of Engineering and Applied Sciences Outstanding Research and Service Award, 1998
  • Invited Foreign Researcher, Japanese Agency of Industrial Science and Technology, 1995
  • Patten Associate Professor Chair in Chemical Engineering, University of Colorado, Boulder, 1993
  • John J. Lee Junior Professorship Chair in Chemical Engineering, Yale University, 1993
  • Senior Faculty Fellowship, Yale University, 1993
  • NSF Presidential Young Investigator Award (1991)


Selected Publications

  • Katayama, DS, Carpenter, JF, Manning, MC, Randolph, TW, Setlow, P, and Menard KP, (2008) “Characterization of amorphous solids with weak glass transitions using high ramp rate differential scanning calorimetry” Journal of Pharmaceutical Sciences Volume 97, Issue 2.
  • Seefeldt MB, Crouch C, Kendrick B, Carpenter, JF, and Randolph, TW (2007) “Specific volume and adiabatic compressibility measurements of native and aggregated recombinant human interleukin-1 receptor antagonist: Density differences enable pressure-modulated refolding” BIOTECHNOLOGY AND BIOENGINEERING 98 (2): 476-485 OCT 1 2007.
  • Thirumangalathu, R, Krishnan, S, Bondarenko, P, Speed-Ricci, M, Randolph, TW, Christians, U, (2007) “Oxidation of methionine residues in recombinant human interleukin-1 receptor antagonist: Implications of conformational stability on protein oxidation kinetics” Biochemistry 46 (21): 6213-6224.
  • Randolph, TW and Carpenter, JF (2007) “Engineering challenges of protein formulations,” AIChE J, 53(8), 1902-1907.
  • Downey, C. D.; Crisman, R. L.; Randolph, T. W.; Pardi, A. (2007) “Influence of Hydrostatic Pressure and Cosolutes on RNA Tertiary Structure” J. Am. Chem. Soc. 129(30); 9290-9291.
  • Ng KY, Zhou HY, Zhang YL, Hybertson B, Randolph T, Christians U (2007). Quantification of isoniazid and acetylisoniazid in rat plasma and alveolar macrophages by liquid chromatography-tandem mass spectrometry with on-line extraction. Journal Of Chromatography B-Analytical Technologies In The Biomedical And Life Sciences 847(2):188-198.
  • Vessely, C., Estey, T, Randolph, TW, Henderson, I, Nayar, R., and Carpenter, JF, (2007) “Effects of Solution Conditions and Surface Chemistry on the Adsorption of Three Recombinant Botulinum Neurotoxin Antigens to Aluminum Salt Adjuvants”, J. Pharmaceutical Science, Journal of Pharmaceutical Sciences, Volume 96, Issue 9, 2375-2389
  • Gabrielson JP, Brader ML, Pekar AH, Mathis KB, Winter G, Carpenter JF, Randolph TW. (2007) “Quantitation of aggregate levels in a recombinant humanized monoclonal antibody formulation by size-exclusion chromatography, asymmetrical flow field flow fractionation, and sedimentation velocity”. J Pharm Sci. 2007 Feb;96(2):268-79.
  • Gabrielson JP, Randolph TW, Kendrick BS, Stoner MR (2007) “Sedimentation velocity analytical ultracentrifugation and SEDFIT/c(s): Limits of quantitation for a monoclonal antibody system”. Anal. Biochem. Feb 1;361(1):24-30. Epub 2006 Nov 28.


Research Interests

Stabilization and Formulation of Therapeutic Proteins- Converting Molecules into Drugs:
Protein-based pharmaceuticals are the fastest-growing class of new drugs. They not only offer promise for treatments to address major health challenges such as cancer, but also a wealth of new engineering problems to solve. Chemical engineers have long been proficient at producing products that meet exacting specifications for chemical purity, but therapeutic proteins now bring additional challenges: these products must not only be highly chemically pure, but also conformationally pure, and must remain so after manufacturing through the drug’s entire shelf-life and delivery to patients. For economic viability, therapeutic protein formulations typically require a shelf life of 18-24 months. Over the course of this time the protein must retain adequate chemical and conformational purity. Meeting the stringent requirements for chemical and conformational stability during shelf life is a daunting task. Most of the common chemical degradation products (especially hydrolysis and oxidation byproducts) are significantly thermodynamically favored versus the desired native state of the protein. Furthermore, the properly folded native state of most proteins is only marginally more stable (the free energy of unfolding, ΔGunf, is about 20-60 kJ/mol) than the unfolded state, and appears to be unstable under most conditions with respect to aggregated forms of the protein.

To slow degradation sufficiently to allow proteins to be used as therapeutic agents, proteins must be placed in a formulation that confers suitable stability against physical and chemical degradation. In addition to stabilizing the pharmaceutically-active protein ingredients, formulation components, or excipients, also must be compatible with their intended use. For example, a formulation intended for parenteral use (e.g., subcutaneous injection) must be sterile, non-toxic and exhibit acceptable viscosity and tonicity. Although these requirements place limits on the types and concentrations of excipients that practically can be used, there are still far too many possible sets of formulations to allow a purely empirical screening approach to be used. The approach that our group takes is to explore fundamental mechanisms of processes that result in degradation and instability of therapeutic proteins. In particular, we use a number of spectroscopic techniques (e.g., FTIR, EPR, NMR, 2D-UV, LALLS, fluorescence spectroscopies) and physical techniques (e.g., analytical ultracentrifugation, titration microcalorimetry, field flow fractionation, mass spectrometry) to understand how solution variables (such as concentration and type of excipients, protein type and concentration, solution ionic strength) and process variables (e.g., agitation) interact to stabilize or destabilize proteins.

Fibrillar aggregates of insulin

Immunogenicity of Protein Therapeutics:

Therapeutic proteins are susceptible to aggregation in response to a wide variety of stresses encountered during their manufacture, storage and delivery to patients. In turn, aggregates of therapeutic proteins may compromise their safety and efficacy. The primary safety concern is that aggregates in therapeutic protein products may induce immune responses, which can have consequences ranging from reduction of product efficacy to patient fatality. Currently it is not well-known what characteristics of protein aggregates are responsible for immunogenicity. We are working to understand how nano- and microparticulate contaminants (including protein aggregates and exogenous microparticles resulting from processing) affect the immune response to therapeutic proteins. In these studies, we rely on physical and spectroscopic methods to characterize aggregate size and structure, and then test how animal models (usually naïve or transgenic mice) respond to parenteral administration of the aggregates.

A related area is our studies of protein-based vaccines. Vaccines offer tremendous benefit to human health, but creation of vaccine formulations that provoke a reliable, protective immune response in a formulation with adequate shelf life is a serious challenge. We are studying the stability of protein structure when adsorbed to relevant surfaces such as the aluminum phosphate salt microparticles that are currently used as adjuvants to enhance immune response.