Flow Cytometry Shared Core

Information about the Flow Cytometry Shared Core Facility

Contact: Theresa Nahreini

Instruments

• BD Accuri C6 Cytometer with Automated, multisampler/Flow Cytometer

​The BD Accuri C6 Cytometer with automated, multisampler is an easy to 
use cell analyzer that supports the use of any brand of tube, including microcentrifuge tubes, and 12 x 75 mm tubes made from polystyrene or polypropylene.  

The 2 laser system consists of the following:
488 nm (FL1 533/30; FL2 585/40; FL3 670 LP)
640 nm (FL4 675/25)

The sampler allows for automation of 48- and 96-well plates and deep plates as well as a 24 tube rack for 12 x 75 mm tubes.

The intuitive software can be mastered in minutes and presentations can be made by a drag and drop of plots into Microsoft Office applications.

• BDFACSCelesta Flow Cytometer Analyzer

​The BDFACSCelesta Flow Cytometer multicolor analyzer allows detection of up to 14 parameters using 3 lasers (405 nm, 488 nm, and 561 nm).  The system has three preset flow rates of LO (12 uL/min), MED (35 uL/min) and HI (60 uL/min) and a data acquisition rate of 25,000 events/s with beads. It operates using BDFACSDiva software to streamline workflow for analysis and presentation applications.

• BD FACSAria Fusion Cell Sorter

​The Special BD FACSAria Fusion™ Cell Sorter within a Biological Safety Cabinet  is built for superior multicolor sensitivity, safety and performance.

The instrument will detect up to 13 parameters, with the following optical emission filters for each of the 4 indicated lasers:

Coherent OBIS 445 nm laser (75 mW):
CFP
AmCyan (505LP; 525/50)
Coherent OBIS 488 nm laser (100 mW):
SSC
FITC, GFP, YFP (495 LP; 515/20)
PerCP-Cy5.5 (685 LP; 710/50)
Coherent Sapphire 561 nm laser (50 mW):
PE (586/15)
mCherry, PE-Texas Red (595 LP; 610/20)
PE-Cy5 (635 LP; 670/30)
PE-Cy7 (750 LP; 780/60)
Coherent OBIS 640 nm laser (40 mW):
APC (670/30)
Alexa 700 (695 LP; 730/45)
APC Cy7 (750 LP; 780/60)

Automated Cell Deposition Unit (ACDU):

This multi-position sorting unit deposits cells onto
slides and into 6, 24, 48, 96, and 384-well plates.Single cell sorting is enhanced with the index sorting function that allows post-sorting review of precise cell
characteristics for each deposited event.

Software: BD FACSDiva 

To achieve superior multicolor performance, the fluidics and optical systems are precisely integrated to maximize signal detection. Innovations include the laser excitation optics, the patented flow cell with gel-coupled cuvette, and the highly efficient patented octagon and trigon detection system. These systems work in unison to achieve unparallelled sensitivity and resolution.