Light Microscopy Core Facility

Information about the Light Microscopy Core Facility

Contact: James Orth

Website: https://www.colorado.edu/lmcf/

Intsruments

• Dell Precision 7910 Analysis PC

The analysis PC is configured with an Intel Xeon 6 core 2.4 GHz processor, 32 GB RAM, NVidia Quadro K4200 4GB video card, and dual 24" monitors.  In addition to the standard free software including ImageJ, FIJI, and Cell Profiler, this PC is equipped with full versions of Nikon NIS Elements (including SIM reconstruction) and 3i Slidebook 6 software.

• Leica DMRXA Upright Widefield Microscope

The upright Leica DMRXA microscope is equipped with a wide range of objectives, filters for four color imaging with a xenon lamp, motorized X, Y, and Z, and a CCD camera.  This instrument is capable of image deconvolution using Slidebook 6 software.

• Nikon E600 Upright Widefield Microscope

The upright Nikon E600 widefield microscope is equipped with a range of objectives and filters for four color imaging using a xenon lamp.  This microscope has a monochrome Nikon Digital Sight DS-Qi camera for fluorescence imaging and a Spot Insight color camera for brightfield imaging (e.g., histology samples).  This instrument has phase contrast capability and a motorized Z drive. 

• Nikon N-SIM structured illumination super-resolution and A1 laser scanning confocal microscope

The inverted Nikon N-SIM/A1 microscope is a combination structured illumination microscopy (SIM) and laser scanning confocal microscope.  SIM imaging utilizes a high sensitivity EM-CCD camera and the A1 confocal contains two highly sensitive GaAsP and two enhanced PMT detectors.  The instrument is equipped with six laser lines (405, 445, 488, 515, 561, and 647 nm) and a wide range of objectives including high NA 20x and 40x, an extra long working distance 40x, and a 100x 1.49 NA.  The Tokai Hit incubation chamber can be used on this microscope.  This microscope can be used for a wide range of applications and is particularly well suited to imaging at super-resolution and confocal imaging of dim samples. 

• Nikon Inverted Spinning Disk Confocal Microscope

The inverted Nikon spinning disk confocal microscope is equipped with four laser lines (405, 488, 561, and 647 nm), a range of objectives, and dual EM-CCD cameras for widefield and confocal imaging.  An additional 405 nm laser can be used for photobleaching or photoconversion imaging.  A Tokai Hit incubation chamber is available for live cell imaging. 

• Zeiss 510 Laser Scanning Confocal Microscope

The inverted Zeiss LSM 510 laser scanning confocal microscope is equipped with three PMT detectors, seven laser lines (405, 458, 477, 488, 514, 543, 633 nm), motorized Z-drive and a wide range of objectives.  This instrument is best suited for confocal imaging of fixed samples.