We measure carbon and oxygen isotopes in air and ice to better understand Earth systems, including the carbon cycle, past climate, and methane.  We excel at measurements of (1) δ13C and δ18O atmospheric carbon dioxide, (2) δ13C of atmospheric methane and local sources of elevated methane, and (3) water from ice cores as well as environmental waters. We provide our analytical services to scientists around the world.

Photo of Bruce Vaughn

Bruce Vaughn
Lab manager

Photo of Sylvia Englund Michel

Sylvia Michel
Assistant lab manager

Photo of Tyler Jones

Tyler Jones
Faculty fellow

Photo of Jianghanyang Li, also known as Ben

Jianghanyang (Ben) Li
Faculty fellow

Photo of Bradley Markle

Bradley Markle
Faculty fellow

Photo of Pieter Tans

Pieter Tans
Faculty fellow emeritus

Photo of Valerie Morris

Valerie Morris
Research staff

Photo of John Ortega

John Ortega
Research staff

Photo of Kevin Rozmiarek

Kevin Rozmiarek
Research staff

Photo of Kerstin Braun

Kerstin Braun
Research staff

Photo of Reid Clark

Reid Clark
Research staff

Photo of Taline Leon

Taline Leon
Research assistant

Photo of Paloma Siegel

Paloma Siegel
Research assistant

  • Chloe Brashear, lab technician 2023
  • Hayley Bennett, MS 2023
  • Alyssa Johnson, lab technician 2021
  • Abigail Thayer, PhD 2021
  • Seth Kurtz, lab technician 2021
  • William Skorski, MS 2020
  • Soisiri Chanin, undergrad 2021
  • Lauren Eng, undergrad 2020
  • Isaac Vimont, PhD 2017
  • Karen Alley, PhD 2017
  • Amy Steiker, MS 2018
  • Caroline Alden, PhD 2013
  • Emily Longano, undergrad 2013

Panorama of the INSTAAR Stable Isotope Lab showing mass spectrometers, preparation stations, gas cylinders, computers, and more

Panorama of the INSTAAR Stable Isotope Lab


Our team

We are a dedicated crew from different scientific backgrounds and experiences, but with common purpose; to understand the Earth's climate system, the carbon cycle, and global change. We work together in the lab and in the field in our shared mission. We also try to have some fun.


Collage of people for the INSTAAR Stable Isotope Lab" Bruce Vaughn, Brad Markle, Sylvia Michel, Valerie Morris, Reid Clark, Chloe Brashear, Seth Kurtz, Kevin Rozmiarek

Some current and past SIL team members, from left to right: Bruce Vaughn, Brad Markle, Sylvia Michel, Valerie Morris, Reid Clark, Chloe Brashear, Seth Kurtz, Kevin Rozmiarek

 Reid Clark, Chloe Brashear, Taline Leon, Sylvia Michel, John Ortega

Current and past members of the greenhouse gas team: Reid Clark, Chloe Brashear, Taline Leon, Sylvia Michel, John Ortega

Members of the SIL team take a tour of Niwot Ridge.

SIL folk take a tour of Niwot Ridge, above INSTAAR's Mountain Research Station.



Blog headlines

  • A send-off for Chloe!
  • An isotope introduction
  • SIL survives the covid times.
  • SIL finds solidarity at GGMT.
  • SIL is flying skyward!

See our blog >


Stable isotopes are a unique tool because they indicate, record, integrate, and trace processes in the global environment. The INSTAAR Stable Isotope Lab partners with researchers from a variety of academic institutions and governmental agencies worldwide in interdisciplinary research into Earth systems.

Rachel Edie changes gas flasks out on Spock, a mass spectrometer that measures stable isotopes of carbon and oxygen in atmospheric CO2, primarily from the NOAA CMDL Cooperative Air Sampling Network. Photo by Ethan Welty.

Rachel Edie changes gas flasks out on Spock, a mass spectrometer that measures stable isotopes of carbon and oxygen in atmospheric CO2 from the NOAA Global Monitoring Laboratory's Global Greenhouse Gas Reference Network. Photo by Ethan Welty.

We measure stable isotopes of carbon dioxide

As the concentrations of greenhouse gases increase, the Earth's climate is changing. The most important anthropogenic greenhouse gas is carbon dioxide - and yet we don't fully understand fluxes of CO2 between the biosphere, the atmosphere, and the ocean.  Stable isotopes are very useful for understanding these exchange processes because of fractionation: when different isotopes of CO2 mover between these different pools at different rates. For instance, because plants strongly discriminate against the heavy isotope of CO2, we can use δ13C-CO2 (this is the 'isotope delta' value) to estimate ocean versus land uptake of carbon dioxide.(Learn more about why isotopes are useful for understanding sources and sinks of carbon!)

The INSTAAR Stable Isotope Lab (SIL) has collaborated with the NOAA Global Monitoring Laboratory Global Monitoring Laboratory's since 1990. This involves measuring δ13C-CO2 and δ18O-CO2 from flasks and programmable flask packages from the Global Greenhouse Gas Reference Network. We proudly maintain the largest dataset of stable isotopes in the world. Our data are available for anyone to use (updates coming soon! Or contact Sylvia Michel for more information. 

Reid loads a pfp in preparation for analysis of methane stable isotopes.

Reid loads a pfp in preparation for analysis of methane stable isotopes.

We measure the stable isotopes of atmospheric methane

Methane is also a very important greenhouse gas, and the atmospheric burden of methane is increasing rapidly for reasons we don't fully understand. Because sources of methane have different isotopic signatures, we can use δ13C-CH4 to use this to test hypotheses about where that increase has come from - and studies suggest that the increase is primarily from a biogenic source, like wetlands or agriculture, though fossil fuel sources may also play a smaller role. Modeling CH4 as well as its isotopes helps us understand the methane budget, and potentially make climate-sensitive policy decisions.

The INSTAAR Stable Isotope Lab (SIL) has been measuring δ13C-CH4 of methane since 1998, and we have the largest collection of data, in the world. We work closely with collaborators around the world making similar measurements to improve our data compatability.

We measure the stable isotopes of oxygen and hydrogen in water from Ice cores.

On Earth's great ice sheets, Greenland and Antarctica, snow accumulates and never melts, creating a repository of precipitation back in time. Stable isotopes of precipitation are a proxy for temperature when the snow fell, making ice cores a rich paleoclimate archives. In addition, ice cores preserve records of atmospheric gases, chemistry, and physical properties, and they are unique for their combination of high resolution and long time scales.  Understanding the climates of the past is essential for predicting the Earth’s responses of human-caused climate change, and ice cores are invaluable in this effort.

3-minute video tour by Tyler Jones of work and life at the North Greenland Eemian Ice Drilling (NEEM) station atop the Greenland ice sheet. The international NEEM team retrieved an ice core from the top of the ice sheet down to bedrock, containing climate records reaching back 115,000 years.

For more than 15 years, SIL has been involved in ice core projects in Greenland, Antarctica, the high-altitude tropics (Ecuador, Peru, Tibet), and other regions. Over this time, SIL has become a world leader in measurement of both deuterium/hydrogen and oxygen-18/16 ratios of ice. In addition to the stable isotope analysis, we are involved with the coring, processing, and modeling/analysis of the data.

We have recently completed ice core projects at NEEM, Greenland, and WAIS Divide, Antarctica. We measured the ice from WAIS Divide on a custom-built continuous melter system connected to a Picarro cavity ring-down mass spectrometer, resulting in higher-than ever measurement resolution on a deep core. We will use this system to measure the core from South Pole, and will develop the next generation core for analysis of the Renland Ice Core.

Water vapor stable isotopes matter.

Turns out it's not just the snow that determines the stable isotopes of water in the ice sheet. The water vapor influences the ice sheet, and the ice sheet influences the water vapor.

There is an awful lot of carbon in permafrost soils.

In a warming planet, will that carbon be released as methane, which is a strong greenhouse gas? The Navigating the New Arctic project, led by Tyler Jones and Bruce Vaughn, investigates how much methane will be released from permafrost soils. Other SIL-folk involved in the project are Kevin Rosmiarek, Valerie Morris, Chloe Brashear, and Taline Leon.

The Mobile Methane Analyze mounted on a white truck with white cab. Photo by Christi Turner.

The Mobile Methane Analyzer. Photo by Christi Turner.

Software visualizing the real-time data collected when methane sampling along roads in Weld County, Colorado. 

Visual display of a mobile methane sampling along roads in Weld County. 


SIL participated in the CU-based, NSF funded Sustainability Research Network called the Air Water Gas project which is aimed at studying the oil and gas industry in the Rocky Mountain West.

The mission of this project was to provide a logical, science-based framework for evaluating the environmental, economic, and social trade-offs between development of natural gas and protection of water and air resources. The project also aims to educate the public and influence the development of policies and regulations governing natural gas and oil development.

Unlike all of our lab-based measurements, Mobile Methane measurements are recorded on the fly as the vehicle drives. The data is plotted onto an interactive map and Landsat image.


Contact & services

Come visit! We are in the southeast corner of SEEC (Sustainability, Energy, and Environment Complex) at 4001 Discovery Dr.

Lab phone: 303 492-5495 

  • We provide analytical services to scientists and institutions around the world.
  • See the list of services below, and contact us with questions you may have.

Information on our measurements
Analysis   Analysis Mode Sample Volume Precision (‰) Cost Per Analysis Time
C & O: carbon dioxide δ13C
dual inlet 400 cc minimum
1 Liter preferred
$60 4-6 wks
C: atm. methane δ13C continuous flow 40 cc minimum
1 Liter preferred
±0.04 $240 4-6 wks
C: soil carbon dioxide δ13C
continuous flow 1 ml minimum
50 ml preferred
Contact us Contact us
H: liquid water & ice δD dual inlet 2ml minimum
15 ml preferred
±0.5 $50 4-6 wks
O: liquid water & ice δ18O dual inlet 2 ml minimum
15 ml preferred
±0.07 $50 4-6 wks
C: organics δ13C continuous flow 10-50mg Carbon required ±0.30 $24 4-6 wks
H: atm. methane δD continuous flow 150 cc minimum ±1.5 Contact us Contact us

  • Sample Volume First line is amount needed for analyses; second line is the desired amount of sample to be submitted, to allow enough for replicate analyses.
  • Precision is the long term reproducibility (one sigma) of a known-unknown.
  • Cost Per Analysis is an estimate for off-campus non-academic research, including commercial parties and governmental agencies. Prices are lower for academic projects and university research. Assistance with interpretation of data is available. We do not attempt to compete with commercial labs.
  • Analysis time is an average turnaround time for a small number of samples (1-50). Actual time may be shorter or longer depending on machine performance, barring mechanical failures beyond our control.


  • We will bill your university/party/agency after the work is completed. (Most universities pay with a purchase order or credit card).
  • We strongly support student projects, both graduate and undergraduate. If you have a limited number of samples, and an even more limited source of funding, we may be able to help.
  • PLEASE DO NOT SEND SAMPLES BY STANDARD US POSTAL SERVICE.  Choose a street delivery service such as FedEx, UPS, etc. We request that you email us with an electronic list (spreadsheet) of sample IDs, so that we can avoid any possibility of data entry typos on our end. If possible, especially with large sample sets, we appreciate some organization of the samples in the shipping container, so we don't have to re-order them.

Standard gases

We also perform calibration of clean dry air cylinders typically filled at the Niwot clean air site by NOAA personnel. Calibrated tanks can be obtained from NOAA Global Monitoring Laboratory, analyzed for mixing ratios of a suite of greenhouse gases, including CO, CO2, N2O, and CH4. For more information, visit the NOAA GML standard-gases web site. Reference cylinders usuallly require analyses made over a period of weeks to verify the stability of the standard air before it can be released and certified for use. Turnaround times can vary, depending on the performance of the cylinder. Typically we make approximately 30 measurements over a period of 6 to 12 weeks. Actual time may be shorter or longer depending on machine and cylinder performance, barring any mechanical failures beyond our control.

Are you interested in being a SIL team member?

Potential graduate students: please reach out to our principal investigators Tyler Jones, Brad Markle, or Ben Li!

Potential undergraduates and Professional Research Assistants: please add your name to this form and email an introduction to Sylvia.

Stable Isotope Lab by the numbers



Full-Time Employees
+ students & postdocs



of operations



in Nature or Science



assoc. with the lab



Square Feet
of freezer space
at -20°C/-4°F



of liquid nitrogen
on demand



Square Feet
of lab space



per year



Our equipment includes

  • Dual-inlet and continuous-flow isotope-ratio mass spectrometers
  • Laser-based cavity ring-down spectrometers.


Collage for instrument Spock, including the mass spectrometer, flask stand, etc

Spock is a Micromass dual inlet mass spectrometer that measures the stable isotopes of carbon and oxygen in atmospheric CO2, primarily from flasks and standard cylinders with the NOAA CMDL Cooperative Air Sampling Network. CO2 is extracted from an air sample by pulling the air through a water trap and into a liquid nitrogen trap. The sample is then heated to expand into the sample bellows of the mass spectrometer. The sample and reference gas are then iteratively allowed into the source where they are ionized, repelled down the flight tube, and separated by isotopic mass. Reliable measurements of CO2 isotopes depends on careful data crunching and quality controls—please contact us if you'd like more information on our procedures.


Collage for instrument T'Pol, including the mass spectrometer, connections to flask packages inside black cases, etc

T'Pol is a GV Isoprime dual inlet mass spectrometer dedicated to running isotopes of CO2 from programmable flask packages. These collect air from aircraft and from tall towers, allowing for sampling along vertical gradients. The sample extraction is almost identical to Spock: CO2 is cryogenically separated from air and expanded into the sample bellows for dual inlet analysis against a CO2 reference gas. Careful monitoring ensures that different instruments measure samples with high accuracy and precision.


Collage for instrument Picard, including the mass spectrometer, connections to air samples, etc

Picard is also a GV Isoprime with a similar extraction system as T'Pol and Spock. Although it can measure samples from flasks and programmable flask packages, it is primarily devoted to measuring air from calibration cylinders. Picard has the capacity to measure CO2 extracted from carbonates, as this is the reference material for CO2 isotopes.


Collage for instrument Troi, including the mass spectrometer, sample preparation, etc

Troi measures carbon isotopes of methane. Since there is not enough methane in a network flask to measure using dual inlet techniques, we rely on continuous flow measurements, where the sample moves on a stream of helium gas. Methane and other gases are frozen on a pre-concentrator of hayesep-D, cryo-focused, and separated on a GC column. The CH4 is combusted to CO2, and then the sample is "sniffed" from an open split into the mass spectrometer.


Collage for instrument Crusher, including the mass spectrometer, pyrolysis furnace, and more

Crusher will soon measure hydrogen isotopes of methane. This measurement is challenging due to the high-temperature pyrolysis furnace which converts CH4 to H2 gas.


Collage for instruments Julian and Phlox, including the mass spectrometers, sample system, and workstations

Julian and Phlox are Picarro cavity ring-down mass spectrometers dedicated to measuring hydrogen and oxygen isotopes of water from discreet samples.


Collage for instrument Kes, including the mass spectrometer, sampler, and more

Kes measures water isotopes from ice-cores. Kes represents is a breakthrough in our ability to obtain high resolution records from ice cores. As the ice is melted, peristaltic pumps move the water into a de-bubbler, splitter (where a portion of the sample is archived), a nebulizer, then vaporized and measured by CRMS.




Overview video (90 sec)

Watch an 90 second overview of the Stable Isotope Lab.  The video will automatically start at the 2:30 mark. This overview is part of a tour of two INSTAAR labs.  The first part of the video gives an overview of the Laboratory for AMS Radiocarbon Preparation and Research.  



Logo for National Oceanic and Atmospheric Administration (NOAA)

National Oceanic and Atmospheric Administration (NOAA). We work closely with NOAA’s Global Monitoring Lab, providing critical isotopic constraints on the sources and sinks of major greenhouse gases and related carbon- climate feedback processes—constraints which are not otherwise available from observations of trace gas concentration alone.

Logo for the National Science Foundation (NSF)

National Science Foundation (NSF). Many of our projects have been supported under the leadership of the National Science Foundation.


We also collaborate with colleagues at many universities, government labs, and other science organizations and communities around the world.