stem-cells-iPSCs-human-training-researchers-BSCsHands-On Human Induced Pluripotent Stem Cell Culture Training

The Stem Cell Research and Technology Resource Center offers hands-on training in the culture of human iPSCs for Basic and Translational Research.

This includes a hands-on iPSC culture workshop suitable for those with prior experience in mammalian cell culture or no prior cell culture experience. The workshop's goal is to quickly give you the skills and knowledge you need so that you can get started independently and confidently growing your own iPSCs and undertaking iPSC-based experiments. Topics include:

  • Maintenance of human iPSCs in feeder-dependent or feeders-free conditions.
  • Preparation of common feeder-free cell culture substrates: Matrigel, Geltrex, CELLStart, and Vitronectin
  • Preparation of common human iPSC media for feeder-dependent and feeders-free conditions.
  • Passaging of human iPSCs (using enzymatic, non-enzymatic solutions and manual techniques)
  • Cryopreservation and thawing of human iPSCs in feeder-dependent or feeders-free conditions.
  • Banking hiPSC 
  • Differentiation, assessment of differentiated human iPSCs, and available kits  
  • Reprogramming methods, demonstrating cells to be iPSCs, gene-editing with CRISPR/Cas9, transfection options, 3D/organoid culture options, and more.
  • Where to purchase human iPSCs and choose lines
  • Sterile technique, preventing contamination, and mycoplasma testing.

stem-cells-human-iPSC-training-researchers

User Fees

Equipment/Service/person

Internal (CU) Rate/person

Non-Profit Rate/person

External Corporate Rate/person

Hour

Stem Cell Culture Training**

$975/complete course

$160.00

*Contact the Director

Hour

Additional Training, Consultation, Writing/Editing, and Other Services $80.00 $160.00 *Contact the Director Hour

* Consult for additional training

* The biosafety cabinet fee includes common cell culture plastics and other necessary supplies (6-, 12-, 24-, and 96-well tissue culture-treated plastic plates; T25 flasks; 2 mL aspirating serological pipets; 1 mL, 5 mL, 10 mL, 25 mL, and 50 mL serological pipets; 15 mL and 50 mL conical tubes; 1.5 mL microcentrifuge tubes; cryovials; 2 μL, 20 μL, 200 μL, and 1000 μL pipette tips; gloves; lab coat usage and cleaning service; Kimwipes; paper towels; pipet-aids and pipette sets [2 μL, 20 μL, 200 μL, and 1000 μL] for each biosafety cabinet; storage space at room temperature and in refrigerators and freezers [boxes provided]; access to -80C freezer storage space; common iPSC solutions [PBS, DMSO, sterile water, 0.5 mM EDTA, Dispase, DMEM/F12 media, Trypsin-EDTA]), as well as use of all Center-maintained equipment (except for fluorescence microscopes) and lab maintenance, including preparing 70% ethanol, autoclaving biohazardous waste and sterile water, ordering and exchanging of compressed gas tanks, Mycoplasma testing, pipette calibrations, biosafety cabinet certifications, and other cleaning and maintenance of equipment and lab space.   

** The training fee includes all handouts, supplies, and consumables (as detailed above), topical lectures, use of dedicated iPSC equipment, iPSCs to practice with, and the one-on-one hands-on training time itself. 

For questions about training and user fees, please contact the Center.