Training

Hands-On human induced Pluripotent Stem Cell (hiPSC) Culture Training

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The Stem Cell Research and Technology Resource Center offers hands-on training in the culture of human iPSCs for Basic and Translational Research. 

The course includes a hands-on iPSC culture workshop suitable for those with prior experience in mammalian cell culture or no prior cell culture experience. The workshop aims to quickly give you the skills and knowledge to start independently and confidently growing your iPSCs and undertaking iPSC-based experiments. Topics include:

•          Isolation and reprogramming of iPSC from PBCs and Fibroblast,

*          Transfection systems.

•          Cell engineering and gene transfer, CRISPR.

•          Maintenance of human iPSCs (feeder-dependent and feeders-free conditions).

•          Expansion (enzymatic, non-enzymatic solutions, and manual techniques) of iPSC (feeder-dependent and feeders-free conditions).

*         A next-generation PSC suspension culture system for optimal expansion and scale-up!

•          Banking (feeder-dependent and feeders-free conditions).

•          Cryopreservation and thawing of iPSCs (feeder-dependent and feeders-free conditions).

*          Characterization and release testing.

*          Mycoplasma testing

*         Cell treatments

•          Preparation of common feeder-free cell culture substrates Matrigel, Geltrex, CELLStart, Vitronectin, etc.

•          Preparation of standard human iPSC media (feeder-dependent and feeders-free conditions).

•          Resource to purchase iPSCs lines and request services.

•          Sterile technique, preventing contamination.

•          Differentiation of iPSCs to NPCs, Neurons, Cardiomyocytes, Hepatocytes, and available kits. Including derivation, culturing, expansion, freezing, thawing, and characterization.

•          3D organoid: derivation, culturing, expansion, freezing, thawing, and characterization.

•          And more!   

 

Workshop on Culturing Primary Cells from Human and Animal Tissues

This workshop provides comprehensive, hands-on training in the isolation, handling, and maintenance of primary cells derived from human and animal tissues. Participants gain practical experience with essential techniques used in modern cell biology and biomedical research, with an emphasis on good laboratory practices, sterility, and reproducibility.

Topics covered include:

  • Tissue processing and cell isolation: Enzymatic and mechanical dissociation methods for obtaining viable primary cells.
  • Aseptic technique and biosafety: Best practices for working safely and effectively with primary cultures.
  • Culture initiation and maintenance: Media preparation, plating densities, substrate selection, and environmental conditions.
  • Monitoring cell health and morphology: Identifying healthy vs. stressed cells, contamination detection, and troubleshooting.
  • Passaging and short‑term expansion: Gentle handling strategies to preserve phenotype and viability.
  • Cryopreservation and recovery: Freezing protocols, thawing techniques, and viability optimization.
  • Ethical and regulatory considerations: Guidelines for working with human- and animal-derived tissues.
  • And more...!
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For questions about training and user fees, please contact the Center.