Hands-On human induced Pluripotent Stem Cell (hiPSC) Culture Training

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The Stem Cell Research and Technology Resource Center offers hands-on training in the culture of human iPSCs for Basic and Translational Research. 

The course includes a hands-on iPSC culture workshop suitable for those with prior experience in mammalian cell culture or no prior cell culture experience. The workshop aims to quickly give you the skills and knowledge to start independently and confidently growing your iPSCs and undertaking iPSC-based experiments. Topics include:

•          Isolation and reprogramming, transfection systems.

•          Cell engineering and gene transfer.

•          Maintenance of human iPSCs in feeder-dependent or feeders-free conditions,

•          Expansion (enzymatic, non-enzymatic solutions, and manual techniques).

•          Banking.

•          Cryopreservation and thawing of iPSCs in feeder-dependent or feeders-free conditions.

•          Differentiation, assessment of differentiated human iPSCs, and available kits  

•          3D and organoid culturing.

•          Characterization and release testing.

•          Preparation of common feeder-free cell culture substrates: Matrigel, Geltrex, CELLStart, and Vitronectin.

•          Preparation of standard human iPSC media for feeder-dependent and feeders-free conditions.

•          Where to purchase human iPSCs and choose lines.

•          Sterile technique, preventing contamination, and mycoplasma testing

•          And more!

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For questions about training and user fees, please contact the Center.