Services

The CU Boulder Stem Cell Research and Technology Resource Center offers CU Researchers and Industries the following services:

Biosafety Cabinets (24/7)

• Biosafety Cabinet with lab supplies and some consumables
•  Biosafety Cabinet without lab supplies and consumables 

*It is allowed to work with any human cell line except human Embryonic Fibroblast nor human Embryonic Stem Cells.

 

Fluorescence Microscopes (24/7)

• Inverted Fluorescence Scanner Microscope (Olympus IX83) with live-cell time-lapse capabilities (Live and fixed cell imaging in monolayer or suspensions, organoids, embryos, organotypic slides, etc.)
• Sterile dissection hood equipped with a dissecting Olympus CKX53 microscope and heated/CO2 stage for work with live cultures and live/fixed images.

 

iPSC lines

• Thaw iPSCs 
• Expand and Freeze iPSCs
• Mycoplasma Testing
• Surface stem cell markers

 

iPS Cell Line Request

The Core has several established human Fluorescently and non Fluorescently Tagged Human Induced Pluripotent Cell Lines for purchase for research purposes. Please contact the director for available cell lines.

 

Miscellaneous

• Cell Proliferation Assay
• Media changes 
• Live culture of iPSC lines
• Fresh Matrigel/Vitronectin/Geltrex pre-coated plates 
• Generating iPSC cell pellet
• Monthly Core usage charge

 

Other Cell Services

• 24/7 Remote Cell Culture Monitoring (Olympus Provi CM20)
• Co2 Shaker with remote controller-Organoid's derivation
• Nucleofector 2b Device – Transfection system
• Olympus IX83 Microscope Training
• Olympus CKX53 Microscope Training
• Olympus Provi CM20 Training
• Consulting

 

Biorepository

• Vial or box for 100 Cryoviales (Price per month or year)

 

Reagents from ThermoFisher (up to 15 % discount. The final cost already includes shipping costs)

• Media
• Kits
• Supplements
• Serum
• Matrices
• Cytokines
• Small molecules
• Free samples
• Free demos

 

Reagents from STEMCELL Technologies  (up to 15 % discount. The final cost already includes shipping costs)

 

Kits and services through Synthego Company (up to 10% discount on products and some services).

Synthego-Synthetic sgRNA Kits: 

• High-efficiency guide RNAs for knockout and knock-in edits
  • CRISPR Design Tool, design top-scoring guide RNAs for gene knockout
  • Inference of CRISPR Edits (ICE) Analysis, a free online tool that provides an easy knockout score
• Gene Knockout Kits v2
  • Using our multi-guide design approach for protein-coding genes in human and mouse genome
• Arrayed CRISPR Screening Libraries 
  • Available in custom, pre-designed, and whole human/mouse genome gene sets

Synthego-​Standard Edits:

• Human iPSCs, ESCs, and Immortalized Cell Lines 
  • Fragment deletion 
  • Knock-ins up to 2,000 bp (Tag / Region Insertion) 
  • Single nucleotide variant changes (SNV)
  • Single, Double, and Triple Gene Knock-out (KO)

Look https://www.synthego.com/legal/product-guarantees to see Synthego's industry-leading guarantees for our cell-editing services.

Type of Edit	Description	Deliverable Type	Turn Around Time
Gene KO	Creating a gene KO using a synthetic guide to make a functional protein knockout. Single, double, triple gene KOs are supported.	Pool	8 – 10 weeks
		Clone	16-20 weeks
SNV Change	Changing a single nucleotide variant (SNV) or single nucleotide polymorphism. It can be either a nucleotide or amino acid change.	Pool	10 – 12 weeks
		Clone	16-20 weeks
Small Tag/ Insertion	Insert a small tag (100 bp or less) into a genome region.	Pool	10 – 12 weeks
		Clone	16-20 weeks
Large Tag / Region Insertion	Insert a large tag or region of interest (2,000 bp or less) into a targeted genome region.	Pool	16-20 weeks
		Clone	20-28 weeks

Synthego-Standard Deliverables for Engineered Cell Projects: 

●    2 independent biological clones or 2 cell pools (2 vials of each: 0.5M cells per vial)

●    2 vials of wild-type isogenic cells mock-transfected pools, passage matched (0.5M cells per vial)

●    Genotypic Analysis and Editing Summary Report 

●    Certificate of Analysis

Synthego-Additional Information:

•  Cell Editing Process
• Learn more about our Halo and Eclipse Platform
• Target Discovery with Arrayed Screening Libraries
•  Engineered Cell Libraries

For pricing information or a quote for a specific project(s), please email Lucia.GayossoMiranda@colorado.edu with the following details: Cell Line, Knock-out, or Knock-iN. If Knock-out, please specify the gene name; if Knock-in,  type of edit: (i.e., SNV, Myc tag, GFP, etc.). Note: times and dates you are available for a discussion

 

Professional Development

• hiPSC training: Over 36 hours of training course. 

It is focused on working with hiPSC in basic and translational research. Lecture topics and hands-on training include iPSC generation and characterization, growth, maintenance, cryopreservation, thawing of hiPSCs, and generation of iPSC-derived cell lines and organoids.

 

Custom Services

Please contact the Core director if you require custom services not listed above. We will do our best to accommodate you!