The CU Boulder Stem Cell Research and Technology Resource Center offers CU Researchers and Industries the following services:

Biosafety Cabinets (24/7)

  • Biosafety Cabinet with lab supplies and some consumables
  •  Biosafety Cabinet without lab supplies and consumables 

*It is allowed to work with any human cell line except human Embryonic Fibroblast nor human Embryonic Stem Cells.

Fluorescence Microscopes (24/7)

  • Inverted Fluorescence Scanner Microscope (Olympus IX83) with live-cell time-lapse capabilities (Live and fixed cell imaging in monolayer or suspensions, organoids, embryos, organotypic slides, etc.)
  • Sterile dissection hood equipped with a dissecting Olympus CKX53 microscope and heated/CO2 stage for work with live cultures and live/fixed images.

iPSC lines

  • Thaw iPSCs 
  • Expand and Freeze iPSCs
  • Mycoplasma Testing
  • Surface stem cell markers

iPS Cell Line Request

The Core has several established human Fluorescently and non Fluorescently Tagged Human Induced Pluripotent Cell Lines for purchase for research purposes. Please contact the director for available cell lines.

Miscellaneous

  • Cell Proliferation Assay
  • Media changes 
  • Live culture of iPSC lines
  • Fresh Matrigel/Vitronectin/Geltrex pre-coated plates 
  • Generating iPSC cell pellet
  • Monthly Core usage charge

Other Cell Services

  • 24/7 Remote Cell Culture Monitoring (Olympus Provi CM20)
  • Co2 Shaker with remote controller-Organoid's derivation
  • Nucleofector 2b Device – Transfection system
  • Olympus IX83 Microscope Training
  • Olympus CKX53 Microscope Training
  • Olympus Provi CM20 Training
  • Consulting

Biorepository

  • Vial or box for 100 Cryoviales (Price per month or year)

Reagents (up to 30% discount. The final cost already includes shipping costs)

  • Media
  • Supplements
  • Serum
  • Matrices
  • Cytokines
  • Small molecules
  • Kits and services through Synthego Company (up to 10% discount on products and some services).

synthego

Synthego-Synthetic sgRNA Kits: 

  • High-efficiency guide RNAs for knockout and knock-in edits
  • Gene Knockout Kits v2
    • Using our multi-guide design approach for protein-coding genes in human and mouse genome
  • Arrayed CRISPR Screening Libraries 
    • Available in custom, pre-designed, and whole human/mouse genome gene sets

Synthego-Standard Edits:

  • Human iPSCs, ESCs, and Immortalized Cell Lines 
    • Fragment deletion 
    • Knock-ins up to 2,000 bp (Tag / Region Insertion) 
    • Single nucleotide variant changes (SNV)
    • Single, Double, and Triple Gene Knock-out (KO)

Look https://www.synthego.com/legal/product-guarantees to see Synthego's industry-leading guarantees for our cell-editing services.

Type of Edit

Description

Deliverable Type 

Turn Around Time

Gene KO 

Creating a gene KO using a synthetic guide to make a functional protein knockout. Single, double, triple gene KOs are supported.

Pool

8 – 10 weeks

Clone

16-20 weeks

SNV Change

Changing a single nucleotide variant (SNV) or single nucleotide polymorphism. It can be either a nucleotide or amino acid change.

Pool

10 – 12 weeks

Clone

16-20 weeks

Small Tag/ Insertion

Insert a small tag (100 bp or less) into a genome region. 

Pool

10 – 12 weeks

Clone

16-20 weeks

Large Tag / Region Insertion

Insert a large tag or region of interest (2,000 bp or less) into a targeted genome region. 

Pool

16-20 weeks

Clone

20-28 weeks

Synthego-Standard Deliverables for Engineered Cell Projects: 

●    2 independent biological clones or 2 cell pools (2 vials of each: 0.5M cells per vial)

●    2 vials of wild-type isogenic cells mock-transfected pools, passage matched (0.5M cells per vial)

●    Genotypic Analysis and Editing Summary Report 

●    Certificate of Analysis

Synthego-Additional Information:

For pricing information or a quote for a specific project(s), please email Lucia.GayossoMiranda@colorado.edu with the following details: Cell Line, Knock-out, or Knock-iN. If Knock-out, please specify the gene name; if Knock-in,  type of edit: (i.e., SNV, Myc tag, GFP, etc.). Note: times and dates you are available for a discussion

 

Professional Development

  • hiPSC training: Over 26 hours of training course. 

It is focused on working with hiPSC in basic and translational research. Lecture topics and hands-on training include iPSC generation and characterization, growth, maintenance, cryopreservation, thawing of hiPSCs, and generation of iPSC-derived cell lines and organoids.

 

Custom Services

Please contact the Core director if you require custom services not listed above. We will do our best to accommodate you!