The Center provides users with a clean, dedicated space specifically for culturing human induced plurupotent stem cells (iPSCs), occupying approximately 1,250 square feet of space on the second floor of Porter Biosciences, on the CU Boulder main campus. The Center is home to four biosafety cabinets, five CO2 and variable oxygen control (tri-gas) incubators, inverted phase contrast microscopes, and a sterile dissection hood equipped with a dissecting fluorescence microscope and heated stage. Additional equipment available to users includes an inverted fluorescence microscope (Olympus IX83) with live-cell time-lapse capabilities, automated fluorescence cell counter, additional phase contrast microscopes, centrifuges, liquid nitrogen cryostorage space, refrigerators, freezers, water baths, and dedicated bench space. Standard iPSC consumables (i.e., pipettes, plasticware, media, substrates, etc.) are readily available to users. Routine mycoplasma testing is performed in-house. Other services currently offered include performing media changes and preparing Matrigel plates for users.
Please contact the Center to find out how you can get started using this shared facility! The Center is available to non-profit academic researchers and for-profit groups as well.