The Center provides users with a clean, dedicated space specifically for culturing human induced pluripotent stem cells (iPSCs), occupying approximately 1,250 square feet of space on the second floor of Porter Biosciences, on the CU Boulder main campus.
We provide:
- 5 Biosafety Cabinets
- 7 CO2 and tri-gas incubators
- Automated Fluorescence Cell Counter
- Inverted Fluorescence Scanner Microscope (Olympus IX83) with live-cell time-lapse capabilities (Live and fixed cell imaging in monolayer or suspensions, organoids, embryos, organotypic slides, and more!)
- Sterile dissection hood equipped with a dissecting Olympus CKX53 microscope and heated/CO2 stage for work with live cultures and live/fix imagine
- Olympus Provi CM20 (imaging and monitoring cell confluence, cell count, and cell culture data records)
- CO2 shaker + remote controller for organoids derivation
- Nucleofector
- Phase contrast microscopes
- Liquid Nitrogen Cryostorage space
- Standard iPSC consumables (i.e., pipettes, plasticware, media, substrates, etc.) are readily available to users.
- Reagents for sale (even 30% less)
- Training of all new users
- hiPSC training
- Microscope training
- Refrigerators & freezers (4OC, -20oC, -80oC)
- 5 Water/Beads bath
- Centrifuges and microcentrifuges
- Plate shakers
- Block heathers (microcentrifuge tubes)
- Mr. Frosty & CoolCell LX Cell Freezing Containers
- Bioengineering space
