stem-cells-training-researchers-human-iPSCs

The Center provides users with a clean, dedicated space specifically for culturing human induced pluripotent stem cells (iPSCs), occupying approximately 1,250 square feet of space on the second floor of Porter Biosciences, on the CU Boulder main campus. 

We provide:

  • 5 Biosafety Cabinets
  • 7 CO2 and variable oxygen control (tri-gas) incubators
  • Automated Fluorescence Cell Counter
  • Inverted Fluorescence Scanner Microscope (Olympus IX83) with live-cell time-lapse capabilities (Live and fix cell imaging in monolayer or suspensions, organoids, embryos, organotypic slides, etc.)
  • Sterile dissection hood equipped with a dissecting Olympus CKX53 microscope and heated/CO2 stage for work with live cultures and live/fix imagine
  • Olympus Provi CM20 into an incubator for imaging and monitoring cell confluence, cell count, and cell culture data records
  • CO2 shaker with remote controller for the derivation of organoids i.e. mini-brains
  • Phase contrast microscopes 
  • Routing Mycoplasma testing 
  • Liquid Nitrogen Cryostorage space
  • Standard iPSC consumables (i.e., pipettes, plasticware, media, substrates, etc.) are readily available to users. 
  • Reagents for sale (even 30% less) 
  • Training of all new users
  • hiPSC training
  • Microscope training
  • Refrigerators, freezers (4OC, -20oC, -80oC)
  • 5 Water/Beads bath
  • Centrifuges and microcentrifuges

Stem cell center user using a microscope to inspect cells.