Specialized Equipment
EVOS 7000
- Outstanding image quality and versatility with a 5-position objective turret, 4-color LED fluorescence and transmitted light channels, and 3.2 MP CMOS color and B/W cameras.
- Fully automated X/Y scanning stage, autofocus, and acquisition routines.
- High-speed image acquisition (scanning, Z-stack, and tile-stitch, 2D/3D- deconvolution, visualization, and analysis).
- Time-lapse live cell imaging (control of temperature, gases, and humidity).
- Image analysis (cell segmentation and quantification with the optional Celleste™ Image Analysis Software).
- High-quality automated fluorescence imaging.
- Full automation of the precision X/Y-stage movement; changing of the 4 LED fluorescent light cubes, 5-position objective turret, focus, and exposure; and switching of the dual camera.
- Can program to run well-plate scanning, time-lapse experiments, and tile-stitch/montage area scans in Z-stack and/or time-lapse modes in your vessel of choice.
- Versatile and highly configurable with a 5-position objective turret and the ability to simultaneously acquire images in four fluorescence channels plus transmitted light.
- An extensive suite of tools for acquisition, visualization, and analysis.
- Smart LED illumination technology.
- Easy to use and reliable!
Olympus IX83
- Fully automated multidimensional observation (X, Y, Z, and time) of live and fixed cells, organoids, embryos, organotypic slices, tissues, etc.
- Z-stack images.
- Automatic focusing with multiple objective lenses. Time-lapse acquisitions can easily be created for high-magnification and multipoint observation.
- Bright field, phase contrast, or fluorescence (DAPI, FITC, TRITC).
- Fast image acquisition and overlay to view samples with multiple fluorescent lenses.
- Automatically scanning multiple points at regular time intervals provides a steady stream of quantitative data in live cultures and organotypic slices.
Videos:
IX83: Trans-mesenteric Migration of Enteric Neural Crest Derived Cells (ENCCs) https://www.olympus-lifescience.com/en/video/ix83_trans_mesenteric/
IX83: Brain Slice Culture Glia Cell https://www.olympus-lifescience.com/en/video/ix83_brain_slice/
IX83: Real-time Imaging for Ring1BYFP/YFP ME https://www.olympus-lifescience.com/en/video/ix83_ring1_byfp_yfp_me/
IX83: Synthetic ERK Activity Propagation by a Light-Switchable System https://www.olympus-lifescience.com/en/video/ix83_synthetic_erk_activity_propagation/
IX83: Light-induced Oscillatory/Sustained Expression of Ascl1 in Neural Progenitor Cellshttps://www.olympus-lifescience.com/en/video/ix83_neural_progenitor_cells/
IX83 Inverted Microscope: Adding Oil to the Objective https://www.olympus-lifescience.com/en/video/ix83-inverted-microscope-adding-oil-to-the-objective/
IX83 Inverted Microscope: Loading a Sample Slide on the Stagehttps://www.olympus-lifescience.com/en/video/ix83-inverted-microscope-loading-a-sample-slide-on-the-stage/
IX83 Inverted Microscope: Focusing and Positioning the Stage Using the U-MCZ Remote Controllerhttps://www.olympus-lifescience.com/en/video/ix83-inverted-microscope-focusing-and-positioning-the-stage-using-the-u-mcz-remote-controller/
Olympus CKX53
- Phase-contrast.
- 4x, 10x, 20x, and 40x magnification.
- Bright field, phase contrast, or fluorescence (DAPI, FITC, TRITC).
- Quickly acquisition and overlapping of images to view samples with multiple fluorescent lenses.
Olympus Provi-scanner
- Automatically scanning multiple points at regular time intervals in culture vessels provides a steady stream of quantitative data about the health and confluency of cultures remotely.
- No need to stain or peel the cell cultures to check cell growth status.
- Remotely alert when the culture approach confluency and is ready for passage.
- Easy storage, reuse, transfer of data, comparison with past results, or data captured under different conditions.
Neon NxT Electroporation System
- Transfect even the most challenging cell types with high efficiency, cell recovery/viability, and reproducibility.
- Transfect cells quickly with an easy three-step workflow, a single buffer kit, and an intuitive user interface.
- Precisely optimize electroporation parameters for different cell types, cell densities, payloads, and electroporation protocol.
- Minimize sample transfer loss and contamination risk.
- Video: https://www.thermofisher.com/us/en/home/life-science/cell-culture/transfection/neon-electroporation-system.html?ef_id=Cj0KCQjw6cKiBhD5ARIsAKXUdyY2g2bL7CucWvZRKTsCWMSO7X7p_nt3mjvIpXoPCkueyPdtlvpTx1QaAvUkEALw_wcB:G:s&s_kwcid=AL!3652!3!652579792520!e!!g!!neon%20electroporation!2058097106!75756882203&cid=bid_clb_tfx_r01_co_cp0000_pjt0000_bid00000_0se_gaw_bt_pur_con&gclid=Cj0KCQjw6cKiBhD5ARIsAKXUdyY2g2bL7CucWvZRKTsCWMSO7X7p_nt3mjvIpXoPCkueyPdtlvpTx1QaAvUkEALw_wcB
Nucleofector 2b Transfection System
- Efficient transfection of hard-to-transfect cell lines and primary cells.
- Easy adjustment and configuration of parameters.
Automated Cell Counter - Countess II FL and Countess 3
- Highly accurate and precise brightfield and fluorescence cell counting.
- Clump cell count.
- Automatic lighting and focus adjustment for optimal contrast and resolution.
- Automatic saving of cell counts and data.
- The software includes convenient built-in pre-dilution and cell-splitting calculators, especially for stem cell culture work.
- Time to count two samples (sides A and B) ~1 minute
Incubator Orbital Shaker CO2
- Organoid growth.
- Permanent conservation inside an incubator, which allows easy access and assembly.
- Easy adjustment and configuration of RPM and time.
- Plates, dishes, and flasks stick to the surface, even when quickly shaken.
Thermocyclers
UV Sterilizing cabinet
Evos Microscope
- Quick and easy observation of cell cultures without the need to use eyepieces.
- Objectives at 4x, 10x, 20x, and 40x.
- Easy change of the aperture to adjust the desired viewing conditions.
- High-quality LED display.
- Easy image acquisition and storage during cell culture work.
Lucetta 2 Luminometer
- Cell proliferation Assay.
- Cytotoxicity Assay.
- Luciferase reporter gene assay.
- Mycoplasma Detection Assay.
ThawSTAR® CFT2 Automated Cell Thawing System
- Thawing Time: Less than three minutes
- Obtain reproducible thawing profiles using a standardized thawing method.
- Eliminate the risk of contamination from water bath-based thawing techniques.
- https://www.youtube.com/watch?v=r2mOaIrt9Cw
E1-ClipTip Bluetooth Electronic Multichannel Pipettes
- Enhanced accuracy and precision. Automate daily 96-and 384-well microplate pipetting
- Two operating options (step-based Matrix or Presets with settings for most common applications)
- Storing 30 of the most common Matrix or Presets protocols with a specific name.
- Customize the main menu view by organizing the icons of preferred order or creating favorite programs and pipetting functions.
- Connect the pipette to the My Pipette Creator app conveniently via Bluetooth**
- Choose a volume range from 0.5μL to 1250μL:
- Single channels
- Multichannel with 8, 12, or 16 channels in 96 and 384 formats
- Adjustable tip spacing multichannel with 6, 8, and 12 channels in 96 and 384 formats.
Thermo Fisher Scientific HERAcell Vios 160i incubators
- Remote temperature and CO2 levels monitoring ensure an optimal culture environment for cell cultures (normoxic and hypoxic conditions).
- Copper interiors provide an antimicrobial surface to avoid contamination and cell death.
- Normoxic or Hypoxic Conditions.
Biosafety cabinets with or without lab supplies and some consumables.
For questions about lab equipment, please contact the Center!