Crystallization

Phoenix Dropsetter

Image of Phoenix Dropsetter Instrument

For setting up crystallizations in multi-well plates; minimum drop size, 100 nanoliter.

Formulatrix Rock Imager

Image of the Dropsetter insturment

Crystallization imagers are installed at 20 °C and 4 °C for imaging crystals in 96-well plates.

Stereo Microscopes

Olympus XZ10 Microscope

We have several manual microscopes installed in all crystallization rooms, including the depicted SZX 10 Olympus microscope equipt with a Lumen 200 Fluorescence Illumination System.

Temperature-Controlled Walk-In Crystallization Rooms

temperature controled crystalisation rooms

We have 3 temperature contolled walk-in crystallization rooms at 4, 20 and 30 ^oC with shelf space for hundreds of crystallization trays. Microscopes are installed in all rooms to facilitate easy Xtal monitoring and Xtal fishing without temperature changes or unnessesary transfer of plates between differnet rooms. In addition we have one 12^oC and one 16 ^oC degree crystallization incubator.

Users should contact Dr.Erbse if they are interested in using the X-ray facility and to schedule hands on training.
All users must complete the online "Radiation Safety Training for X-Ray Machine Users" before they can enter the X-ray facility to receive training from Dr.Erbse.
All users must receive hands-on training, from Dr.Erbse, before they are allowed to use the instruments independently. The in person training consists of one training session. If possible, the training is done using user samples.

After your online and hands on training is completed, you will be given access to the Google calendars for the instruments and access to the X-ray facility. You must sign up for instrument time using the Google calendars.

All the instruments in the X-ray core are located in the basement of JSCBB in the C-Wing, room C1B51, on East Campus. Card access is required at all times. Users will only be given access after completing both online and in person trainings.

Users are expected to provide all consumables specific to their experiment.
Groups must pay for any parts or repairs due to damage by neglect or carelessness (If you break it - you bought it!).
Cost for repairs/parts/services resulting from normal wear will be split between all user groups.
Please Note - Since repairs/service from normal wear might not be necessary every year, depending on use, these costs might come quite some time after the use dates.

Users must sign up using the Google calendar for the drop setter. During use, users must sign the paper log at the instrument.
Users must stay with the robot at all times while it is going through a protocol.
Users must perform the "after run” wash protocol directly after they are done or if they are leaving for more than one hour.
Failure to follow these rules will result in loss of the privilege to use the drop setter.

Users must sign up using the Google calendar for the imaging system.
Please do not schedule automatic imaging. Instead start imaging manually.
Check that plates are returned from imaging to their positions in the plate hotel correctly and that no collision occurred.
Remove plates no longer needed from the plate hotel.
Delete images no longer needed from the computer.

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Crystallization

Phoenix Dropsetter

Formulatrix Rock Imager

Stereo Microscopes

Temperature-Controlled Walk-In Crystallization Rooms