The Biomolecular X-Ray Crystallography Lab in the Department of  Biochemistry provides resources for crystallization and structure determination of biological macromolecules and complexes. The first goal of the Biomolecular X-Ray Crystallography Lab is to support research in the Department of Biochemistry. We are happy to extend our help to researchers from the broader CU community, if time and availability allow us too.

Three controlled-temperature rooms (20 °C, 4 °C and 30 °C) are available for crystallization experiments.  We have a Phoenix dropsetter to set up crystal trays, two RockImager system for automatic crystal imaging (at 20 °C and 12 °C) and several microscopes for manual imaging.

Two data collection systems, with cryo-cooling, are available for crystal characterization, cryoprotectant screening, and in-house data collection. One data collection system consists of a Rigaku MM007 rotating anode x-ray generator with Varimax optics, a Raxis IV++ image plate detector, and an Oxford cryostream 800. The second system is a XtaLAB MM003 equiped with a MicroMax-003 Microfocus sealed tube X-ray generator system, a AFC11 Partial-χ, 4-axis goniometer, a Dectris PILATUS 200K 2D hybrid pixel array detector and an Oxford cryostream 700.

Resources are available for remote data collection at synchrotrons, as well as for in-house crystallographic computation and model-building.