Welcome to the Central Analytical Mass Spectrometry Facility and W.M. Keck Foundation Proteomics Resource
The Central Analytical Laboratory at the University of Colorado at Boulder serves as a resource offering analytical and instructional expertise in mass spectrometry. We offer a wide selection of mass spectrometry services to the research community within the University system and at-large, with the aim of providing scientists with practical training and access to the state-of-the-art instrumentation they need to develop and meet their research interests.
The facility is located in room C1B90 of the Jennie Smoly Caruthers Biotechnology Building and currently houses five mass spectrometry systems that provide unique and complementary mass spectrometry capabilities:
Most instruments are interfaced with UPLC or HPLC instruments. Our Orbitrap mass spectrometers are interfaced with 2D nanoAcquity UPLCs for multidimensional high-resolution separations. For small molecules, we provide accurate mass determination and complex mixture analysis (metabolomics). We specialize in all aspects of protein mass spectrometry, from protein identification in simple mixtures to large collaborative projects that include label-free and stable isotope quantitative proteome profiling, post-translational modification mapping, protein-protein interaction analysis of affinity-based isolations, organelle proteomics, protein crosslinking analysis, and hydrogen/deuterium exchange for protein structural analysis. We provide consultation on experimental design, which is especially important for large proteomics studies, and have significant computational resources and software tools available for data processing, visualization, and interpretation.
We provide routine sample analysis performed by laboratory personnel, and also offer training for open access to some instruments including the Thermo ISO LT GCMS, Synapt G2 HDMS, Agilent 6120 LCMS, and 4000 Q-trap QQQ instruments.
Listed below are the types of analyses we routinely perform on submitted samples. Please contact us prior to submission of samples requiring special handling or analysis.