Publications
Cryopreparation of cells for electron tomography. O’Toole, E.T., Giddings, Jr., T.H., and Winey, M. Cold Spring Harb Protoc. 2017 Mar 1;2017(3). doi: 10.1101/pdb.prot085589. PMID: 28250212
Building cell Structures in Three Dimensions: Electron tomography methods for budding yeast. O’Toole, E.T., Giddings, Jr., T.H., and Winey, M. Cold Spring Harb Protoc. 2017 Mar 1;2017(3). doi: 10.1101/pdb.top077685. PMID: 28250232
Conventional transmission electron microscopy. Winey M., Meehl J.B., O'Toole E.T., Giddings T.H. Jr. Mol Biol Cell. 2014 Feb;25(3):319-23. doi: 10.1091/mbc.E12-12-0863. PMID:24482357
Computer-assisted image analysis of human cilia and Chlamydomonas flagella reveals both similarities and differences in axoneme structure. O'Toole E.T., Giddings T.H. Jr., Porter M.E., Ostrowski L.E. Cytoskeleton (Hoboken). 2012 Aug;69(8):577-90. doi: 10.1002/cm.21035. Epub 2012 May 22. PMID: 22573610
"Tips and tricks" for high-pressure freezing of model systems. McDonald K., Schwarz H., Müller-Reichert T., Webb R., Buser C., Morphew M. Methods Cell Biol. 2010;96:671-93. doi: 10.1016/S0091-679X(10)96028-7. PMID:20869543
Electron tomography and immuno-labeling of Tetrahymena thermophila basal bodies. Giddings T.H. Jr., Meehl J.B., Pearson C.G., Winey M. Methods Cell Biol. 2010;96:117-41. doi: 10.1016/S0091-679X(10)96006-8. PMID: 20869521
Chlamydomonas cryopreparation methods for the 3-D analysis of cellular organelles. O'Toole E.T. Methods Cell Biol. 2010;96:71-91. doi: 10.1016/S0091-679X(10)96004-4. PMID: 20869519
High pressure freezing, electron microscopy and immuno-electron microscopy of Tetrahymena thermophila basal bodies. Meehl J.B., Giddings T.H. Jr., and Winey M. Methods Mol Biol. 2009;586:227-41. doi: 10.1007/978-1-60761-376-3_12. PMID: 19768433
Silver enhancement of Nanogold particles during freeze substitution for electron microscopy. Morphew M., He W., Bjorkman P.J., and McIntosh J.R. J Microsc. 2008 May;230(Pt 2):263-7. doi: 10.1111/j.1365-2818.2008.01983.x. PMID: 18445156
Understanding microtubule organizing centers by comparing mutant and wild-type structures with electron tomography. O’Toole E.T., Giddings T.H. Jr., and Dutcher S.K. Methods Cell Biol. 2007;79:125-43. doi: 10.1016/S0091-679X(06)79005-7. PMID: 17327155
Recent advances in high-pressure freezing: equipment- and specimen-loading methods. McDonald K.L., Morphew M., Verkade P., Müller-Reichert T. (2007). Methods Mol Biol. 369:143-73. PMID: 17656750
3D immunolocalization with plastic sections. Morphew M.K. (2007). Methods Cell Biol. 2007;79:493-513. doi: 10.1016/S0091-679X(06)79019-7. PMID: 17327171.
Freeze-substitution protocols for improved visualization of membranes in high-pressure frozen samples. Giddings TH. J Microsc. 2003 Oct;212(Pt 1):53-61. PMID:14516362
Electron tomography of yeast cells. O'Toole E.T., Winey M., McIntosh J.R., and Mastronarde D.N. (2002) Methods Enzymol. 2002;351:81-95. doi: 10.1016/s0076-6879(02)51842-5. PMID: 12073377
Using rapid freeze and freeze-substitution for the preparation of yeast cells for electron microscopy and three-dimensional analysis. Giddings T.H. Jr., O'Toole E.T., Morphew M., Mastronarde D.N., McIntosh J.R., Winey M. Methods Cell Biol. 2001;67:27-42. doi: 10.1016/s0091-679x(01)67003-1. PMID: 11550475
Computer-assisted analysis of flagellar structure. O’Toole E.T., Mastronarde D.N., McIntosh J.R., and Porter M.E. 1995. Meth. Cell Biol. 1995;47:183-91. doi: 10.1016/s0091-679x(08)60808-0. PMID: 7476486