Methods

Methods and Other Resources

Cryopreparation of cells for electron tomography. Yeast Protocols, CSHL Press, Submitted. O’Toole, E. T., Giddings, Jr., T. H., and Winey, M. (2014) Protocols for 3-D EM of yeast:

Building cell Structures in Three Dimensions: Electron tomography methods for budding yeast. Yeast Protocols, CSHL Press, Submitted. O’Toole, E. T., Giddings, Jr., T. H., and Winey, M. (2014)

Conventional transmission electron microscopy. Winey M, Meehl JB, O'Toole ET, Giddings TH Jr. Mol Biol Cell. 2014 Feb;25(3):319-23. doi: 10.1091/mbc.E12-12-0863. PMID:24482357

Computer-assisted image analysis of human cilia and Chlamydomonas flagella reveals both similarities and differences in axoneme structure. O'Toole ET, Giddings TH Jr, Porter ME, Ostrowski LE. Cytoskeleton (Hoboken). 2012 Aug;69(8):577-90. doi: 10.1002/cm.21035. Epub 2012 May 22. PMID:22573610

"Tips and tricks" for high-pressure freezing of model systems. McDonald K, Schwarz H, Müller-Reichert T, Webb R, Buser C, Morphew M. Methods Cell Biol. 2010;96:671-93. doi: 10.1016/S0091-679X(10)96028-7. PMID:20869543

Electron tomography and immuno-labeling of Tetrahymena thermophila basal bodies. Giddings TH Jr, Meehl JB, Pearson CG, Winey M. Methods Cell Biol. 2010;96:117-41. doi: 10.1016/S0091-679X(10)96006-8. PMID: 20869521

Chlamydomonas cryopreparation methods for the 3-D analysis of cellular organelles. O'Toole ET. Methods Cell Biol. 2010;96:71-91. doi: 10.1016/S0091-679X(10)96004-4. PMID:20869519

High pressure freezing, electron microscopy and immuno-electron microscopy of Tetrahymena thermophila basal bodies. Meehl, J. B., Giddings, Jr., T. H., and Winey, M. (2009) Methods in Molecular Biology, 586:227-241. PMID: 19768433

Silver enhancement of Nanogold particles during freeze substitution for electron microscopy. Morphew, M. He, W., Bjorkman, P.J., and McIntosh, J.R. (2008) J Microscopy, 230:263-267. PMID: 18445156

Understanding microtubule organizing centers by comparing mutant and wild-type structures with electron tomography. O’Toole, E.T., Giddings, T.H. Jr., and Dutcher, S.K. (2007) Methods Cell Biol. 79, 125-143. PMID: 17327155

Recent advances in high-pressure freezing: equipment- and specimen-loading methods. McDonald KL, Morphew M, Verkade P, Müller-Reichert T. (2007). Methods Mol Biol. 369:143-73. PMID: 17656750

3D immunolocalization with plastic sections. Morphew MK. (2007). Methods Cell Biol. 79:493-513. PMID: 17327171

Freeze-substitution protocols for improved visualization of membranes in high-pressure frozen samples. Giddings TH. J Microsc. 2003 Oct;212(Pt 1):53-61. PMID:14516362

Electron tomography of yeast cells. O'Toole, E. T., Winey, M., McIntosh, J. R., and Mastronarde, D. N. (2002) Methods Enzymol. 351:81-95.

Using rapid freeze and freeze-substitution for the preparation of yeast cells for electron microscopy and three-dimensional analysis. Giddings TH Jr, O'Toole ET, Morphew M, Mastronarde DN, McIntosh JR, Winey M. Methods Cell Biol. 2001;67:27-42. No abstract available. PMID:11550475

Computer-assisted analysis of flagellar structure. O’Toole, E.T., D.N. Mastronarde, J.R. McIntosh, and M.E. Porter. 1995. Meth. Cell Biol. 47, 183-191. PMID;7476486

Sample Preparation for High Pressure Freezing

Practical Methods in High Pressure Freezing - Mary Morphew download