Schedule Our Equipment

Transmission Electron Microscopes

FEI Tecnai F30

Cameras/detectors:  Gatan K2 Summit (4k) direct electron detector, Gatan OneView IS (4k) CCD

Applications:  Plastic/thick section electron tomography & Cryo-electron tomography, CryoTEM, single particle analysis screening and data acquisition, High-resolution TEM

 

FEI Tecnai F20

  • Cameras/detectors:  Gatan K3 (4k) direct electron detector, Gatan US4000 (4k) CCD

    Applications:  Plastic/thick section electron tomography & Cryo-electron tomography, CryoTEM, single particle analysis screening and data acquisition

FEI Tecnai T12 Spirit

Cameras/detectors:  AMT 2k x 2k side-mounted CCD  (fast scanning), FEI Eagle 4k x 4k CCD (higher resoltuion)

Applications:  plastic/thin section imaging, immunoEM, negative stain imaging, nanoparticles/material samples

Other Microscopic Equipment

Inspect cryo-stage for use in cryo Correlative Light Electron Microscopy (CLEM) as well as live-cell imaging prior to fixation.

Sample Preparation Equipment

Wohlwend compact 02 high pressure freezer.Cryofixation tool capable of generating specimens for cryo-sectioning, freeze-substitution and freeze-etching.

Other Sample Preparation Equipment

Leica Automatic Freeze-Substitution Systems (AFS)

Plunge freezers:

  • FEI Vitrobot Mark IV
  • Manual plunge freezer (custom design)

Ultramicrotomes:

  • Leica Ultracut UCT (plastic)
  • Leica UC6

Electron Microscopy Sciences Carbon Evaporator

EMITEC Glow Discharge Unit

Computer Work Stations:

  • SPA processing
  • Electron tomography processing