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Odorizzi Lab
College of Arts and Sciences
Odorizzi Lab

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Protocols

  • Yeast Transformation
  • Bacterial Transformation
  • DNA Miniprep (Alkaline Lysis)
  • Yeast Whole-Cell Lysis
  • Yeast Spheroplast Preparation
  • FM 4-64 Labeling of Yeast
  • Yeast Random Spore Analysis
  • Ubiquitin Western Blotting
  • Ubiquitin immunoprecipitation
  • Preparation of Competent BL21
  • Preparation of Competent XL1-Blue
  • Preparation of Yeast Genomic DNA
  • GST Pulldown from BL21 Extract
  • Immunofluorescence of Yeast
  • Yeast Genomic Library Construction
  • Protease & Phosphatase Inhibitors
  • Sorvall RC-M120EX Manual
  • Restriction Digest Analysis of DNA
  • 5X Orange G & 1-kb Ladder
  • PCR (Taq)

Odorizzi Lab

Email me at Odorizzi@Colorado.EDU

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