Throughout the academic year, the Department of Biochemistry invites professors from other universities and institutes to speak at the University of Colorado Boulder. These seminars provide an opportunity for faculty and students to learn about exciting current research at other universities.
Dr. Kazuhiro Maeshima
Friday, February 28 | 1:30 pm | JSCBB B231
Talk Title: Single nucleosome imaging reveals that active RNA polymerase II globally constrains genome chromatin
Host: Dr. Karolin Luger
Dr. Eva Nogales
Wednesday, March 4 | 3:30 – 4:30pm | JSCBB A115 - Butcher Auditorium
Talk Title: Challenging Protein Complexes that Regulate the Expression of our Genes: Visualizing themin Action using Cryo-EM
Host: Biophysics Students
TFIID functions as a central hub in therecognition of the core promoter sequences and neighboring chromatin marks andloads the TATA-box binding protein (TBP) onto the DNA. Ourstudies have shown that TFIID undergo dramatic conformational transitions thatare linked to its binding to DNA. While dealing with the complexity of TFIID’sconformational landscape has been challenging, our studies have now led to afull description of the subunit architecture of TFIID and to a model of how thestructure and dynamics of the complex are used to load TBP onto DNA only whenthe core promoter is recognized, leading to the proper recruitment of Pol II atthe transcription start site.
PRC2 methylateslysine 27 of histone H3 and is essential for cellular differentiation anddevelopment. Our structure of human PRC2 in complex with JARID2 and AEBP2showed how AEBP2 stabilizes the full complex, and how the two cofactors mimicthe binding of histone H3 tails. We also described the interaction of PRC2 witha dinucleosome, showing the double engagement of consecutive nucleosomes by thecomplex for different linker lengths. Our recent analysis using differentpost-transcriptional modifications in substrate nucleosomes point to aninteresting interplay between different histone modifications and cofactors inthe regulation of PRC2 enzymatic activity.
Undergraduate Research Symposium
Wednesday, March 11 | 3:30 – 4:30pm | JSCBB A115 - Butcher Auditorium
Host: Dr. Jeffrey Cameron