Office: JSCBB B222
Lab: JSCBB B250
PhD: Biomedical Sciences, Advisor: Jeoung Sook Shin, PhD. UCSF, 2008-2013
Postdoctoral Fellow: Immunology and Infectious Diseases, Advisor: Rick Brown, MD. Genentech, Inc., 2014-2016
Postdoctoral Fellow: Cell Biology, Advisor: Daniel Finley, PhD. Harvard Medical School, 2016-2019
Areas of Expertise
Cell Signaling, Proteins and Enzymology
Understanding Ubiquilins: Facilitators of protein degradation
The ubiquitin-proteasome system is essential to eukaryotic life through the regulation of protein degradation. Ubiquitinated proteins bind to the proteasome via ‘intrinsic ubiquitin receptors’ – subunits of the proteasome cap which promote docking of ubiquitinated substrate to the proteasome for subsequent degradation. In animal models, these subunits are essential to proper development and survival. In addition to intrinsic receptors, mammals also express ‘extrinsic ubiquitin receptors’; these proteins are not subunits of the proteasome but are capable of simultaneously and reversibly binding ubiquitinated proteins and the proteasome. The largest family of extrinsic ubiquitin receptors are called Ubiquilins (Ubqlns), which facilitate degradation of a poorly defined subset of ubiquitinated proteins. Since their discovery, multiple Ubqlns have been implicated in cancer and neurodegenerative disease. In particular, mutations in Ubqln2, which is highly expressed in neurons and muscle, were recently identified as the cause of an X-linked, dominant form of familial Amyotrophic Lateral Sclerosis (fALS).
Recent work has shown that Ubqlns play a crucial role in the ubiquitin-proteasome system, which is central to cellular health, but major questions remain: do Ubqlns regulate the same clients regardless of cell type or stimulus? How does the accumulation of these clients impact cellular health? And how do these clients lead to in vivo pathology when Ubqln function is lost? My laboratory aims to address these crucial questions using unbiased, mass-spectrometry based proteomics paired with in vivo and cell biological methods.
- Whiteley, A.M.*, Prado, M.A.*, de Poot, S.A.H., Paulo, J.A., Ashton, M., Dominguez, S., Weber, M., Syzpyt, J. Jedrychowski, M.P., Easton, A., Gygi, S., Kurz, T., Monteiro, M.J., Brown, E.J., Finley, D. (2020) Global proteomics of Ubqln2-based murine models of ALS. BioRxiv, https://doi.org/10.1101/2020.02.22.956524.
- Wu, J.J., Cai, A., Greenslade, J.E., Higgins, N.R., Fan, C., Le, N.T.T., Tatman, M., Whiteley, A.M., Prado, M.A., Dieriks, B.V., Curtis, M.A., Chaw, C.E., Siddique, T., Faull, R.L.M., Scotter, E.L., Finley, D., Monteiro, M.J. (2020) ALS/FTD mutations in UBQLN2 impede autophagy by reducing autophagosome acidification through loss of function. PNAS Jun 8; online ahead of print.
- Whiteley, A.M., Prado, M.A., Peng, I., Abbas, A.R., Haley, B., Paulo, J.A., Reichelt, M., Katakam, A., Sagolla, M., Modrusan, Z., Lee, D-Y., Roose-Girma, M., Kirkpatrick, D. S., McKenzie, B.S., Gygi, S.P., Finley, D., Brown, E.J. (2017) Ubiquilin1 promotes antigen-receptor mediated proliferation by eliminating mislocalized mitochondrial proteins. eLife 6: e26435.
- Greer, A.M., Matthay, M.A., Kukreja, J., Bhakta, N.R., Nguyen, C.P., Wolters, P.M., Fahy, J.P., Woodruff, P., Fahy, J.V., Shin, Js. (2014) Accumulation of myeloid DCs in inflammatory diseases of the lung. PLoS One 9 (6):e99084.
- Greer, A.M., Wu, N., Putnam, A., Woodruff, P.W., Kinet, J.P., Shin, Js. (2014) FcεRI expressed in human dendritic cells and monocytes mediates cellular entry of circulating IgE contributing to serum IgE clearance. The Journal of Clinical Investigation 124 (3): 1187-1198.