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Resources

Basic Microscopy Links

  • Microscopy links & search engines
  • Help with sample preparation, image acquisition and analysis!
  • Fluorescence & spectra viewers
  • Software download links
  • Standard operating procedures

The University of Colorado Boulder Library Search Engine is great for locating primary literature and text books for research. 

These short courses by iBiology Microscopy are designed for beginners.

Microforum has a wealth of information and is recommended for more advanced users. 

There is also some fun microscopy information in the links below:

  • Imaris - An introduction to 3D visualization & analysis techniques for microscopy data
  • Nikon's MicroscopyU 
  • Zeiss Education
  • Olympus Resource Center
  • Leica Science Lab
  • Chroma Technology Corp
  • Hamamatsu Knowledge and Hamamatsu Camera Simulator
  • iScopeCalc :: Mobile Microscope Calculator
  • University of Colorado at Boulder | Geometric Optics Phet Simulation

 

Image Processing and Data Analysis

  • Matlab on Image Processing
  • Wolfram Alpha on Images
  • Fiji (Is Just ImageJ) - Search Engine
  • Life Technologies Molecular Probes School of Fluorescence
  • Guides for ethical image processing and various handbooks

Mounting Medium

Facility recommendations:

  • Prolong Gold
  • Prolong Diamond
  • Prolong Glass

 

 

 

 

  • Life Technologies SpectraViewer
  • Fluorescent Protein Database - FPbase
  • CiteAB: Antibody database search engine
  • Chroma Spectra Viewer
  • Semrock SearchLight
  • U Arizona - Spectra Database
  • Olympus FluoView Resource Center Dual Probes
  • BD Biosciences Fluorescence Spectrum Viewer
  • Clontech Fluorescent Proteins
  • UNC Michael Hooker Microscopy Facility Table of Fluorochromes

If you have not stumbled across the University Software Downloads and Licensing site, I highly recommend checking out the software that is available for students and staff.

Either way - here are a few well-known image viewers and advanced computer languages for image processing and analysis:

  • Fiji (Is Just ImageJ)
  • Vaa 3D
  • Leica LAS X
  • 3i Slidebook Viewer
  • Nikon Elements Viewer
  • Wolfram Alpha Mathematica (University Site License)
  • Matlab (University Site License)
    • Open Microscopy Bio-Formats for Matlab

Please learn and follow the Standard Operating Procedures listed below while working in the LMCF.

  • Live Cell Handling and Transport SOP

 

 

Light Microscopy Core Facility

Porter Biosciences, B047, B049 and B051                                                                                                                    347 UCB, Boulder CO, 80309
James.Orth@Colorado.EDU
(303) 492-5955

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