Please be aware that this is a common use facility that is utilized by many researchers who expect the equipment to be in proper working order at the beginning of their session. Microscopes are expensive to repair and time spent on maintenance is at the expense of the users. Please be courteous to others and be careful when operating the microscopes.
Signing Up for Equipment
Users can only sign up for equipment one week in advance or within the week. For complex experiments, special arrangements can be made with the Director. Use for every system must be documented in the Excel log sheet located on the Desktop (even if it is just to copy some files). If you log on to the PC, log it in the Excel sheet. If there is no Excel entry, you will be billed at imaging rates.
- Labs are limited to 6 hours of use per day between 8:00 AM - 6:00 PM. Consult the Director if you need to reserve more time on the microscope.
- Do not book more time than you need; abuse will result in billing of unused time.
- Image acquisition takes priority over image analysis. Please use the Hulk for data analysis.
- Start your session punctually. Repeat over-scheduling will result in billing for the full scheduled time, not just hours used.
- If you need to cancel your session, contact the Director. Reservations in the Campus Calendar can be deleted as long as they are deleted before the scheduled time begins.
- Repeated “no-shows” will result in billing of all scheduled time and possible suspension of user privileges.
- You are responsible for making sure the equipment was turned off by the previous user. This is especially important if you cancelled your reservation the same day or just before your start time. Lack of proper shut down for any equipment will result in billing for the entire time the equipment was on.
Using the Microscopes
The LMCF requires users to have a University IdentiKey or a facility personal present for temporay use. Keep all light sources on standby whenever possible to prolong their life. At the end of your session, check the MCDB Calendar; if someone will be utilizing the instrument, please leave it on and log out of your IdentiKey account, otherwise shut down the instrument. Contact the next user if you finish early. Turn each microscope on or off as described; don't turn on components you won't use. Arc lamps must be kept on for 30 minutes at a minimum and need to cool for 60 minutes before being reignited. The life of the lasers and mercury arc bulbs are not infinite; do not leave the microscopes on overnight.
No alcohol or mind altering drugs may be used by anyone using the LMCF; if the Director suspects this is the case, you will be asked to leave the facility. Horse play and practical jokes will not be tolerated at any time. Do not leave any specimens or samples in the facility, dispose of them in your lab. Properly clean up your workspace and any oil objectives you used. Lack of proper facility edict will not be toloarted and privillages to use the facility will be revoked. If you are not sure how to properly clean an objective or operate the equipment please ask for help. In general - when in doubt ASK FOR HELP!
Anyone imaging live samples must obey their principal investigators lab safety protocol and standard operating procedure. Standard Operating Procedure for transporting live human and animal-derived cell lines to the LMCF, handling them in the LMCF and transporting them from the LMCF when finished.
The LMCF is not responsible for data storage. Any images left on the computer hard-drives are unsafe and may be deleted. Store your images on a server or move your data to other storage devices at the end of your session. The MCDB department's server is known as Collie. Please contact the departments Information Systems Manager - Erik Hedel to request access and information on Collie. Finally, please don't leave an excessive amount of data on the facility computers; they can quickly get over-loaded by the volume of images produced.