Super-Resolution

Lasers:

Lauch: LUNV6, high-output.

Excitation at: 405, 445, 488, 515, 561, 647 (nm)

Emission (Chroma) and molecules imaged:

• Emission filter: 425-475 (nm): DAPI, Hoechst, DyLight-405, Alexa405, etc.
• Emission filter: 467.5-502.5 (nm): CFP, cerulean, etc. 
• Emission filter: 500-550 (nm): Alexa488, FITC, EGFP, Opal520, etc. 
• Emission filter: 521.5-554.5 (nm): YFP, Venus, etc. 
• Emission filter: 575-625 (nm): Alexa555, 568, 594, TxRed, mRFP, mCherry, TdTomato, Opal570, etc. 
• Emission filter: 650-720 (nm): Alexa647, Cy5, Opal620, etc. 
• DIC imaging possible (laser scanning confocal only)

Filters for widefield:

DAPI, GFP, RFP, Brightfield

N-SIM certified objectives:

• 100x 1.49NA OIL SR Apo TIRF WD 0.12 (mm)

• 60X 1.27NA Water SR Plan Apo WD 0.17 (mm)

Standard objectives:

• 4x 0.2NA Plan Apo λ WD 20 (mm)
• 10x 0.45NA Plan Apo λ WD 4 (mm)
• 20x 0.75NA Plan Apo VC λ WD 1 (mm)
• 40x 0.6NA S Plan Fluor ELWD 3.6-2.8 (mm)
• 40x 0.95NA Plan Apo λ WD 0.25-0.17 (mm)

Available upon request: 

• 60x 1.40NA Plan Apo OIL VC λ WD 0.13 (mm)

Laser Scanning Confocal:

• 2 x GaAsP high-sensitivty PMTs (CFP, GFP, YFP, RFP)
• 2 x multi-alkali PMTs (DAPI, far-red)

N-SIM:

Andor iXon X3 EM-CCD 512 X 512 16 bit

• TOKAI-HIT Cell Incubator 1 x 35 (mm) dish or chamber side (coverslip bottom)
• Motorized X, Y, Z and separate Mad City Labs Piezo Z
• Multi-point, time-lapse, optical sectioning, large-area montage with stitching
• Increase power over imaging depth
• SOLA Light engine
• Nikon Perfect Focus System

• NIS Elements 5.21.03; 64 bit

• Thor's User Guide
• Sample Preperation 

THOR - Nikon SIM/A1