Imaging Systems

Typhoon 5 imager

Typhoon 5

Please acknowledge SIP and the NIH funding (1S10OD034218-01) in publications and presentations ( posters, talks) if you use the Typhoon 5.

The Typhoon 5 is a multi-mode, high-sensitivity imaging platform that produces digital images of radioactive, ﬂuorescent, or chemiluminescent samples. It has blue (488 nm), green (532 nm), red (635 nm), near IR (685nm) and far IR (785nm) excitation wavelengths and a wide choice of emission filters (IP, P 390BP, Cy2 525BP20, Cy3 570BP20, Cy5 670BP30, IRshort 720BP20, IRlong 825BP30, Cy2 LPB515, Cy3 LPG550, Cy5 LPR660) enable imaging of an extensive variety of fluorophores. Automated multi-colour fluorescence scanning allows multiplexing of multiple targets in the same sample. It can be used to scan phospho storage screens, gel sandwiches, agarose and polyacrylamide gels, membranes, microplates and even microarrays.

In addition, we have ImageQuant TL, an easy-to-use general image analysis software for data evaluation. It offers 4 different analysis modes for a) 1-D electrophoresis gels, b) dot blots, microplates, and basic arrays, c) colony counting and measurement of 2-D spots and d) a toolbox to analyze gels with user-defined objects.

LAS 4000

The LAS 4000 is a sensitive imager for chemiluminescence, fluorescence and white light applications. It can be used to capture high sensitivity images of agarose and polyacrylamide gels, western blot membranes and agar plates. It has an advanced Fujifilm™ CCD camera and a bright, wide-aperture FUJINON™ F0.85 lens providing exceptional sensitivity for chemiluminescence. It can capture quantitative images at a resolution of up to 6.3 Megapixels. Responses are highly linear and enable precise quantitation across four orders of magnitude.

In addition we have ImageQuant TL, an easy-to-use general image analysis software for data evaluation. It offers 4 different analyses modes for a) 1-D electrophoresis gels, b) dot blots, microplates, and basic arrays, c) colony counting and measurement of 2-D spots and d) a toolbox to analyze gels with user-defined objects

AlphaImager Mini

The AlphaImager Mini gel documentation system provides easy to use, high-performance imaging for a range of UV and colorimetric applications. It has a UV transiluminater with two wave lenghts (302 and 365nm) and two intesity settings. The camera is a 1.4 Megapixel camera. We have an orange filter for EthBr imaging. It has a white light conversion screen that can be used for trans white light ilumination. The Alpha Imager Mini s mainly used for imaging of DNA and protein gels.

All three imaging systems are easy and intuitive to use. It is ok for new users to be trained by someone in their lab, who uses them routinely. Users can also contact Dr. Erbse for help and training on the imaging systems.

Users can sign up for instrument time on EMS. Users must be connected via VPN, if not on campus. After connecting, users can log in with their Identikey username and password. Detailed step-by-step instructions for the sign-up process can be found here. If you need help, please reach out to the SIP director Dr. Erbse. In addition, users must sign the Excel log sheets on the desktops of the PCs to document their instrument usage.

The imagers are located in C381

Regular user groups are expected to opt into the pool and pay an monthly share that goes towards the general up keep of the Shared Instruments Pool. For detailed information please contact Dr. Annette Erbse.
Users are expected to provide all consumables specific to their experiment.
Groups must pay for any parts or repairs due to damage by neglect or carelessness (If you break it - you bought it!).
Cost for repairs/parts/services resulting from normal wear will be split between all user groups.
Please Note - Since repairs/service from normal wear might not be necessary every year, depending on use, these costs might come quite some time after use.
For more infromation see Usage Policies.

Users must clean the stages after use.
Bring water, Ethanol and Kim wipes for cleaning.
Exposures longer than 30 min should not be done during normal working hours. Please time you experiments so that you can do them early or late in the day.
Do not cut on the glass stage.

Users must clean the stages after use, especially the glass fluorescence stage.
Users must bring water, Ethanol and Kim Wipes for cleaning (only absolute 100% alcohol should be used on the glass stage). Lower-quality EtOH can leave fluorescent contaminations.
The large, mounted phospho storage screens can only be used with the Dige stage, please ask for help.
Glass plates should never be placed directly on the glass stage. Use the stage with the adjustable frame to hold your glass plates.
Do not cut on the glass stage.

Users must clean the light box and the conversion screen after use.
Users must bring water, Ethanol and Kim wipes for cleaning.
The AlphaImager Mini runs Windows XP and cannot be connected to the internet. Please scan flash drives for PC and MAC viruses before you use them to retrieve your data.

The Shared Instruments Pool depends on acknowledgements to thrive. For more information on how to acknowledge the Shared Instruments Pool and/or it's personnel, click here or go to the acknowledgment tab.

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Imaging Systems

Typhoon 5

LAS 4000

AlphaImager Mini