Rapid mixing, chemical quench-flow devices can be used to determine fast reaction rates or single turnover rates of enzymatic reactions and to isolate reaction intermediates. In contrast to stopped-flow experiments employing fluorescence, for circular dichroism or absorption spectroscopy no optician output is necessary to monitor the reactions. Instead, reactions are started in a mixing chamber and quenched after a set amount of time by the addition of a chemical quenching reagent. The shorts time points accessible are governed by the dead time of mixing and are in the millisecond range. Substrates, products or intermediates present at the time of quench can be analyzed and quantified by different methods, for example by HPLC, gel electrophoresis, scintillation counting or mass spectrometry to name a few.
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Exciting Possibilities in Biology, Biochemistry and Biophysics
Measure fast enzyme kinetics without suitable optical output
Users have to receive hands-on training from Dr.Erbse, before they are allowed to use the instruments independently. Interested users should contact Dr. Erbse to schedule training. The training will cover sample preparation, performing measurements, cleaning the Quench -Flow Apparatus. Training will be done employing a standard reaction (Base catalyzed hydrolysis of 2,4 dinitrophenyl acetate) in addition to an actual sample provided by the user.
After your training is completed you will be invited to join the RFQ-3 Google Calendar. To reserve RFQ-3 time use the Google Calendar to sign up with your name and lab. Please be sure to avoid booking times that overlap with other users.
The RFQ-3 is easily movable and will be set up in the lab of the interested user. This is especially important for experiments with radioactive labeled samples in order to keep the radioactive work in the designated radioactive working arear of the users.
Regular user groups are expected to opt into the pool and pay an monthly share that goes towards the general up keep of the Shared Instruments Pool. For detailed information please contact Dr. Annette Erbse.
Users are expected to provide all consumables specific to their experiment.
Users are expected to pay for repairs or parts that are necessary due to damage caused by their carelessness or neglect. This includes replacing the titration syringe should it be broken or the needle bent.
Cost for repairs/parts/services resulting from normal wear will be split, based on the time used, between all user groups.
Please Note -
Since repairs/service associated with normal wear might not be necessary every year, depending on use, the cost might come quite some time after use.
