High-Throughput Screening

Our lab uses a novel system for high-throughput screening of protein degradation in the cell. Instead of relying on the cumbersome method of radiolabeling, cycloheximide treatment, and Western blotting, we utilize photoconvertible fluorescent reporters and a custom-engineered LED array. After protein is photoconverted in live cells via LED illumination, the extinction of fluorescent signal is detected over time either by plate reader, flow cytometry, or microscopy.