ES and Primary Cell Culture

The proteome changes significantly depending on the cell type being observed, which can significantly influence our results. Our lab uses a variety of cell culture systems, including human ES cells and murine primary cells, to understand how the proteome changes in neurons and in lymphocytes. We differentiate human ESCs which have been genetically modified to lack UBQLN1, UBQLN2, or UBQLN4, into neurons for our studies of how UBQLNs influence neurodegenerative disease. We also obtain primary murine lymphocytes to understand how UBQLN loss influences their proliferation and protein synthesis.