Model schematic for sticky and slippery obstacles.

Effects of soft interactions and bound mobility on diffusion in crowded environments: a model of sticky and slippery obstacles

June 12, 2017

There are many biological systems in which tracer particles diffuse in a crowded environment. We implemented a lattice Monte Carlo model to analyze the effects of binding kinetics, bound motion, and obstacle size on tracer diffusive in the presence of soft obstacles.Read more »
Model cartoon

Kinesin-5-independent mitotic spindle assembly requires the antiparallel microtubule crosslinker Ase1 in fission yeast

May 17, 2017

Pushing forces generated by microtubule polymerization are sufficient to promote spindle pole separation and the assembly of bipolar spindle in the absence of molecular motors.Read more »
Model schematic.

Analytical structure, dynamics, and coarse-graining of a kinetic model of an active fluid

March 6, 2017

One of the simplest active suspensions with complex dynamics is a suspension of immotile "Extensor" particles that exert active extensile dipolar stresses on the fluid in which they are immersed.Read more »
Spindle images.

Physical determinants of bipolar mitotic spindle assembly and stability in fission yeast

Jan. 20, 2017

Microtubules, motors, and cross-linkers are important for bipolarity, but the mechanisms necessary and sufficient for spindle assembly remain unknown. We describe a physical model that exhibits de novo bipolar spindle formation.Read more »

Molecular determinants of tubulin's C-terminal tail conformational ensemble

Aug. 19, 2016

The disordered C-terminal tails of tubulin are biologically important but difficult to study. We describe a method to isotopically label tubulin for structural studies by NMR of the C-terminal tails.Read more »
Model cartoon

Contributions of microtubule dynamic instability and rotational diffusion to kinetochore capture

June 24, 2016

Recent work has found that microtubule rotational diffusion about minus-end attachment points contributes to kinetochore capture in fission yeast, but the relative contributions of dynamic instability and rotational diffusion are not well understood.Read more »
Cell images

Kinesin-8 effects on mitotic microtubule dynamics contribute to spindle function in fission yeast

April 28, 2016

To better understand the role of kinesin-8 proteins in mitosis, we have studied the effects of deletion of the fission-yeast kinesin-8 proteins Klp5 and Klp6 on chromosome movements and spindle length dynamics.Read more »
Phase diagram

Phase-plane analysis of the totally asymmetric simple exclusion process with binding kinetics and switching between antiparallel lanes

April 3, 2016

Inspired by experiments on the motion of kinesin-4 motors on antiparallel microtubule overlaps, we analyze a model incorporating the TASEP on two antiparallel lanes with binding kinetics and lane switching.Read more »
Filaments and motor image

Microscopic origins of anisotropic active stress in motor-driven nematic liquid crystals

Nov. 23, 2015

We study a physical model of filaments, crosslinking motors, and static crosslinkers to dissect the microscopic mechanisms of active stress generation in a two-dimensional system of orientationally aligned rods.Read more »
Simulation snapshot

Multiscale modeling and simulation of microtubule/motor protein assemblies

Oct. 7, 2015

Microscopic motor activity causes polarity-dependent interactions between motor proteins and microtubules in reconstituted cytoskeletal active matter.Read more »

Pages

Pages