We develop a computational model of fission-yeast mitosis using a course-grained Brownian Dynamic framework in conjunction with a force-dependent kinetic Monte Carlo algorithm to replicate the biorientation and segregation of chromosomes.
Our collaborative paper with Phong Tran's lab, "Kinesin-5-independent mitotic spindle assembly requires the antiparallel microtubule crosslinker Ase1 in fission yeast," was published by Nature Communications .
Our paper "Contributions of microtubule dynamic instability and rotational diffusion to kinetochore capture" was published in final form today by the Biophysical Journal and highlighted on the Biophysical Journal website.
Microtubules, motors, and cross-linkers are important for bipolarity, but the mechanisms necessary and sufficient for spindle assembly remain unknown. We describe a physical model that exhibits de novo bipolar spindle formation.
Recent work has found that microtubule rotational diffusion about minus-end attachment points contributes to kinetochore capture in fission yeast, but the relative contributions of dynamic instability and rotational diffusion are not well understood.