Thumbnail image from Maguire 2019 BPJ submission

Bound-state diffusion due to binding to flexible polymers in a selective biofilter

Aug. 25, 2019

We demonstrate that bound mobility via tethered diffusion can be engineered into a synthetic gel using protein fragments derived from the nuclear pore complex.

Thumbnail of the nuclear pore complex from Maguire et al. 2019

Design principles of selective transport through biopolymer barriers

July 30, 2019

We develop a model motivated by features of the nuclear pore complex (NPC) which provides a framework to control binding-induced selective transport in bipolymeric materials.

Thumbnail image for Wall and Hough 2018 BiophysJ

In-Cell NMR within Budding Yeast Reveals Cytoplasmic Masking of Hydrophobic Residues of FG Repeats

April 19, 2019

In-cell NMR in S. cerevisiae is a novel way of looking at disordered proteins in a crowded cellular environment. The methodology has been developed here, which could be broadly applicable to other protein systems. In an example, the non-specific interactions of the FG Nup construct, FSFG-K, with the cytoplasm is similar to previously found bacterial interactions using NMR relaxation techniques. This work was recently published in Biophysical Journal.

Model schematic for sticky and slippery obstacles.

Effects of soft interactions and bound mobility on diffusion in crowded environments: a model of sticky and slippery obstacles

June 12, 2017

There are many biological systems in which tracer particles diffuse in a crowded environment. We implemented a lattice Monte Carlo model to analyze the effects of binding kinetics, bound motion, and obstacle size on tracer diffusive in the presence of soft obstacles.

Spindle images.

Physical determinants of bipolar mitotic spindle assembly and stability in fission yeast

Jan. 20, 2017

Microtubules, motors, and cross-linkers are important for bipolarity, but the mechanisms necessary and sufficient for spindle assembly remain unknown. We describe a physical model that exhibits de novo bipolar spindle formation.

Thumbnail image for Wall et al. 2016 ACS Chem. Biol. paper

Molecular determinants of tubulin's C-terminal tail conformational ensemble

Aug. 19, 2016

The disordered C-terminal tails of tubulin are biologically important but difficult to study. We describe a method to isotopically label tubulin for structural studies by NMR of the C-terminal tails.

Cell images

Kinesin-8 effects on mitotic microtubule dynamics contribute to spindle function in fission yeast

April 28, 2016

To better understand the role of kinesin-8 proteins in mitosis, we have studied the effects of deletion of the fission-yeast kinesin-8 proteins Klp5 and Klp6 on chromosome movements and spindle length dynamics.

Filaments and motor image

Microscopic origins of anisotropic active stress in motor-driven nematic liquid crystals

Nov. 23, 2015

We study a physical model of filaments, crosslinking motors, and static crosslinkers to dissect the microscopic mechanisms of active stress generation in a two-dimensional system of orientationally aligned rods.

Phase diagram

Hysteresis, reentrance, and glassy dynamics in systems of self-propelled rods

Sept. 30, 2015

Non-equilibrium active matter made up of self-driven particles with short-range repulsive interactions exhibits collective motion and nonequilibrium order-disorder transitions.

Thumbnail image from Hough et al 2015 elife

The molecular mechanism of nuclear transport revealed by atomic-scale measurements

Sept. 15, 2015

Loren E Hough, Kaushik Dutta, Samuel Sparks, Deniz B Temel, Alia Kamal, Jaclyn Tetenbaum-Novatt, Michael P Rout , David Cowburn (2015). eLife 2015;4:e10027 DOI: 10.7554/eLife.10027 Nuclear pore complexes (NPCs) form a selective filter that allows the rapid passage of transport factors (TFs) and their cargoes across the nuclear envelope, while...

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