Thumbnail image for Wall and Hough 2018 BiophysJ

In-Cell NMR within Budding Yeast Reveals Cytoplasmic Masking of Hydrophobic Residues of FG Repeats

April 19, 2019

In-cell NMR in S. cerevisiae is a novel way of looking at disordered proteins in a crowded cellular environment. The methodology has been developed here, which could be broadly applicable to other protein systems. In an example, the non-specific interactions of the FG Nup construct, FSFG-K, with the cytoplasm is similar to previously found bacterial interactions using NMR relaxation techniques. This work was recently published in Biophysical Journal.

Model schematic for sticky and slippery obstacles.

Effects of soft interactions and bound mobility on diffusion in crowded environments: a model of sticky and slippery obstacles

June 12, 2017

There are many biological systems in which tracer particles diffuse in a crowded environment. We implemented a lattice Monte Carlo model to analyze the effects of binding kinetics, bound motion, and obstacle size on tracer diffusive in the presence of soft obstacles.

Spindle images.

Physical determinants of bipolar mitotic spindle assembly and stability in fission yeast

Jan. 20, 2017

Microtubules, motors, and cross-linkers are important for bipolarity, but the mechanisms necessary and sufficient for spindle assembly remain unknown. We describe a physical model that exhibits de novo bipolar spindle formation.

Molecular determinants of tubulin's C-terminal tail conformational ensemble

Aug. 19, 2016

The disordered C-terminal tails of tubulin are biologically important but difficult to study. We describe a method to isotopically label tubulin for structural studies by NMR of the C-terminal tails.

Cell images

Kinesin-8 effects on mitotic microtubule dynamics contribute to spindle function in fission yeast

April 28, 2016

To better understand the role of kinesin-8 proteins in mitosis, we have studied the effects of deletion of the fission-yeast kinesin-8 proteins Klp5 and Klp6 on chromosome movements and spindle length dynamics.

Filaments and motor image

Microscopic origins of anisotropic active stress in motor-driven nematic liquid crystals

Nov. 23, 2015

We study a physical model of filaments, crosslinking motors, and static crosslinkers to dissect the microscopic mechanisms of active stress generation in a two-dimensional system of orientationally aligned rods.

Phase diagram

Hysteresis, reentrance, and glassy dynamics in systems of self-propelled rods

Sept. 30, 2015

Non-equilibrium active matter made up of self-driven particles with short-range repulsive interactions exhibits collective motion and nonequilibrium order-disorder transitions.

Schematic of nuclear pore complex

The molecular mechanism of nuclear transport revealed by atomic-scale measurements

Sept. 15, 2015

We used nuclear magnetic resonance spectroscopy to show that FG repeats are highly dynamic IDPs, stabilized by the cellular environment. Fast transport is supported because the rapid dynamics of transport factor-FG interactions.

model cartoon

Microtubule depolymerization by the kinesin-8 motor Kip3p: a mathematical model

April 30, 2009

Proteins from the kinesin-8 family promote microtubule depolymerization, a process thought to be important for the control of microtubule length in living cells.