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In-Cell NMR within Budding Yeast Reveals Cytoplasmic Masking of Hydrophobic Residues of FG Repeats

April 19, 2019

In-cell NMR in S. cerevisiae is a novel way of looking at disordered proteins in a crowded cellular environment. The methodology has been developed here, which could be broadly applicable to other protein systems. In an example, the non-specific interactions of the FG Nup construct, FSFG-K, with the cytoplasm is similar to previously found bacterial interactions using NMR relaxation techniques. This work was recently published in Biophysical Journal.

BPS meeting logo

Group members presented posters at the BPS annual meeting

Feb. 21, 2018

Christopher Edelmaier, Laura Maguire, Jeffrey Moore, and Loren Hough presented posters on their work at the Biophysical Society annual meeting in San Francisco.

Allison Whited joined Hough lab

Aug. 1, 2017

We would like to welcome Allison Whited to the Hough lab! Allison is a new postdoc in our group beginning August 2017. She comes to us most recently from Oak Ridge National Lab where she has been a postdoc for the last two years studying the structure of lipid membranes...

Mean square displacement curves showing Fickian and anomalous diffusion.

Michael Stefferson's paper accepted by Physical Biology

June 15, 2017

Michael Stefferson's paper "Effects of soft interactions and bound mobility on diffusion in crowded environments: a model of sticky and slippery obstacles" was accepted for publication by Physical Biology.

Model schematic for sticky and slippery obstacles.

Effects of soft interactions and bound mobility on diffusion in crowded environments: a model of sticky and slippery obstacles

June 12, 2017

There are many biological systems in which tracer particles diffuse in a crowded environment. We implemented a lattice Monte Carlo model to analyze the effects of binding kinetics, bound motion, and obstacle size on tracer diffusive in the presence of soft obstacles.

Spindle images

Spindle assembly modeling paper published by Science Advances

Jan. 20, 2017

Robert Blackwell's paper "Physical determinants of bipolar mitotic spindle assembly and stability in fission yeast" was published by Science Advances .

Spindle images.

Physical determinants of bipolar mitotic spindle assembly and stability in fission yeast

Jan. 20, 2017

Microtubules, motors, and cross-linkers are important for bipolarity, but the mechanisms necessary and sufficient for spindle assembly remain unknown. We describe a physical model that exhibits de novo bipolar spindle formation.

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Zach Gergely's paper published by Molecular Biology of the Cell

Nov. 7, 2016

Zachary Gergely's paper "Kinesin-8 effects on mitotic microtubule dynamics contribute to spindle function in fission yeast" was published in final form by Molecular Biology of the Cell.

Portrait of Loren

Loren Hough receives NIH MIRA grant

Oct. 22, 2016

Congratulations to Loren Hough, who received an NIH MIRA grant for new investigators. This 5-year, $1.8 million award will support his lab's research on tubulin tails. News coverage of this award has been posted at the physics department website.

Molecular determinants of tubulin's C-terminal tail conformational ensemble

Aug. 19, 2016

The disordered C-terminal tails of tubulin are biologically important but difficult to study. We describe a method to isotopically label tubulin for structural studies by NMR of the C-terminal tails.

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