David L. Allen

Integrative Physiology; Member of the Center for Neuroscience

Department of Integrative Physiology
Room C373 Ramaley
Campus Box 354
Boulder, CO 80309-0354

email: allendl@Colorado.EDU
Phone: (303) 492-3175
FAX: (303) 492-0811
Website: http://www.colorado.edu/intphys/faculty/allen.html

Dr. David Allen is an assistant professor in the Department of Integrative Physiology. He received both his Bachelor’s of Science degree in Kinesiology and his doctorate in Physiological Science from the University of California, Los Angeles. Prior to starting his current faculty position in Integrative Physiology he did postdoctoral research in the Department of Human Genetics at the University of Michigan Medical School and in the Department of Molecular, Cellular, and Developmental Biology at the University of Colorado, Boulder.

Dr. Allen’s research focuses primarily on the molecular biology and genetics of skeletal muscle gene expression. In particular, his lab uses cell culture, plasmid DNA injection, and transgenic animals to elucidate the cis- and trans-regulatory elements responsible for the expression of genes such as myosin heavy chain, myostatin, insulin-like growth factor, matrix metalloproteinase-9, and the neuronal nicotinic acetylcholine receptor. The study of these genes is organized into four main projects in Dr. Allen’s lab: (1) understanding the signaling pathways that confer fiber-specific and exercise-sensitive gene expression on different myosin heavy chain isoforms; (2) identifying the role of transcriptional regulation of the anti-growth factor myostatin and the autocrine growth factor IGF-I in muscle hypertrophy and atrophy; (3) elucidating the role of matrix metalloproteinases, and polymorphisms in these genes, in exercise-induced muscle damage and repair in both mice and humans; (4) understanding the contribution of genetics to individual differences in susceptibility to nicotine addiction by identifying functional polymorphisms in candidate neural genes.

Selected Publications:

Allen DL Sartorius CA, Sycuro LK, Leinwand LA. Different pathways regulate expression of the skeletal myosin heavy chain genes. Journal of Biological Chemistry, 276: 43524-43533, 2001.

Allen DL, Leinwand LA. Intracellular calcium and myosin isoform transitions. Calcineurin and CaM kinase pathways regulate preferential activation of the IIa myosin heavy chain promoter. Journal of Biological Chemistry 277: 45323-45330, 2002.

Allen DL, Teitelbaum D, Kurachi K. Growth factor stimulation of matrix metalloproteinase expression and myoblast migration and invasion in vitro. American Journal of Physiology 284: C805-C815, 2003.

McCall GE, Allen DL, Haddad F, Baldwin KM. Transcriptional regulation of IGF-I expression in skeletal muscle. American Journal of Physiology 285: C831-839, 2003.