Cells and Microscopy

 

Required Web Sites

Lab # 1:

Microscope Alignment for Koehler Illumination
http://www.microscopyu.com/tutorials/java/kohler/index.html

Linear Measurements (Micrometry)
http://www.microscopyu.com/articles/formulas/measurements.html

Eyepiece Reticle Calibration
http://www.microscopyu.com/tutorials/java/reticlecalibration/index.html

Working Distance and Parfocal Length
http://www.microscopyu.com/articles/formulas/formulasworkingparfocal.html

Molecular Expressions' Digital Video Gallery - Pond Life. Contains amoeba, euglena and paramecium movies and much more.
http://micro.magnet.fsu.edu/moviegallery/pondscum.html

Amoeba Videos
http://micro.magnet.fsu.edu/moviegallery/pondscum/protozoa/amoeba/index.html

Paramecium Videos
http://micro.magnet.fsu.edu/moviegallery/pondscum/protozoa/paramecium/index.html

Amoebas are more than just blobs – Casual and informative introduction to amoeba with the best photographs of Amoeba proteus that I have seen. Don’t miss the “Locomotion and Feeding” page.
http://www.microscopy-uk.org.uk/mag/artsep01/amoeba.html

Lab # 2:

Phase Contrast Microscopy Introduction
http://www.microscopyu.com/articles/phasecontrast/phasemicroscopy.html

Principles of Phase Contrast Microscopy
http://www.microscopyu.com/articles/phasecontrast/phasehome.html

Specimen Contrast in Optical Microscopy
http://www.microscopyu.com/articles/formulas/specimencontrast.html

Phase Contrast Microscope Configuration
http://www.microscopyu.com/articles/phasecontrast/phaseconfiguration.html

Phase Contrast Microscope Alignment
http://www.microscopyu.com/tutorials/java/phasecontrast/microscopealignment/index.html

Immersion Oil and Refractive Index
http://www.microscopyu.com/tutorials/java/objectives/immersion/index.html

Recommended Links

DIC Microscopy

Comparison of Phase Contrast and DIC Microscopy
http://www.microscopyu.com/tutorials/java/phasedicmorph/index.html

Differential Interference Contrast Microscopy
http://www.microscopyu.com/articles/dic/dicindex.html

Reflected Light DIC Microscopy
http://www.microscopyu.com/articles/dic/reflecteddic.html

DIC Microscope Component Alignment
http://www.microscopyu.com/tutorials/java/dic/dicalignment/index.html

Sites for the curious

Cell Movies and Images and Information

Molecular Probes Gallery - lots of pictures of structures and organelles within cells.
http://www.probes.com/servlets/gallery/

Protista Tutorials from Cornell - actually movies.
http://biog-101-104.bio.cornell.edu/BioG101_104/tutorials/protista.html

Stages of Mitosis
http://www.itg.uiuc.edu/technology/atlas/structures/mitosis/

Studying Cells Tutorial - Information on the Scientific Method, Size in Biology and the Major Events in Cell Biology
http://www.biology.arizona.edu/cell_bio/tutorials/cells/cells.html

Prokaryote, Eukaryote and Viruses Tutorial - from University of Arizona Biology Project http://www.biology.arizona.edu/cell_bio/tutorials/pev/main.html

Cells.de - Online service for Cell Biology - Cell Structure
http://www.cells.de/cellseng/medienarchiv/index_medien.htm

Protozoa - General

Medical Protozoology
http://www.tulane.edu/~wiser/protozoology/index.html

Protistologistís Home Pages
http://mtlab.biol.tsukuba.ac.jp/WWW/Servers/Protistologists.html

Protist Information Server
http://mtlab.biol.tsukuba.ac.jp/olddefault.html

The Micropolitan Museum ‚ Presented by 'Institute for the Promotion of the Less than One Millimeter' really nice photos
http://www.microscopy-uk.org.uk/micropolitan/index.html

Bio 332: Ciliates Part II ‚ Tetrahymena and Paramecium Details
http://www.zoology.ubc.ca/courses/bio332/Labs/CiliateProject/ciliate2/titlepage2.htm

Ciliated Protozoans Gallery
http://ebiomedia.com/gall/ciliates/index.html

Amoeba

Molecular Expressions Digital Video Gallery: Amoeba
http://micro.magnet.fsu.edu/moviegallery/pondscum/protozoa/amoeba/index.html

Measuring Amoeba Movement
http://www.silkentent.com/gus1911/Amoeba2.htm

Amoeboid Movement
https://www.arches.uga.edu/~beauw/amoeba.html

Amoeba proteus
http://mtlab.biol.tsukuba.ac.jp/WWW/PDB/Images/Sarcodina/ap/indexE.html

Introduction to Microscopy – Amoeba proteus in different spotlights
http://www.microscopy-uk.org.uk/intro/illum.html

Euglena

The Euglenoid Project
http://bio.rutgers.edu/euglena/

Euglena
http://www.nies.go.jp/chiiki1/protoz/morpho/flagella/euglena.htm

Protist Images - euglena
http://mtlab.biol.tsukuba.ac.jp/WWW/PDB/Images/Mastigophora/Euglena/

Paramecium

Molecular Expressions Digital Video Gallery: Paramecium
http://micro.magnet.fsu.edu/moviegallery/pondscum/protozoa/paramecium/index.html

The Arsenal of Paramecium
http://www.expasy.ch/spotlight/articles/sptlt003.html

Kung Lab Paramecium Research
http://www.molbio.wisc.edu/kung/para.html

Osmoregulation in Paramecium
http://www.douglas.bc.ca/biology/project/paramecium/

Feeding and Food Vacuole Processing
http://www.zoology.ubc.ca/courses/bio332/Labs/FVprocedure.htm

BioMEDIA classics – Paramecium Nice short explanations and fantastic photos
http://ebiomedia.com/gall/classics/Paramecium/paramecium1.html

Micscape Image of the Month – Paramecium
http://www.microscopy-uk.org.uk/mag/artmar99/marimg99.html

Imperialistic Paramecium
http://www.lazylabs.org/paramecium.html

Sonneborn Paramecia – site is sort-of self-explanatory
http://www.improb.com/airchives/paperair/volume6/v6i2/sonneborn-6-2.html

Tetrahymena

Team Tetrahymena
http://www.stolaf.edu/depts/biology/tetrahymena/

Tetrahymena Genome Project
http://lifesci.ucsb.edu/~genome/Tetrahymena/

Introduction to Tetrahymena Genetics
http://www.lifesci.ucsb.edu/~genome/Tetrahymena/genetics.htm

The Ciliate Resource Archive
http://www.uoguelph.ca/%7Eciliates/

TIGR Tetrahymena thermophila gene index
http://www.tigr.org/tdb/tgi/ttgi/

Dictyostelium

Dictyostelium Cinema
http://www-biology.ucsd.edu/~firtel/movies.html

Dictyostelium Virtual Library
http://dictybase.org

Physarum

Introduction to the "Slime Molds"
http://www.ucmp.berkeley.edu/protista/slimemolds.html

Cellular Structures

Actin and Microtubules
http://www.itg.uiuc.edu/technology/atlas/structures/actin/

Endoplasmic Reticulum
http://www.itg.uiuc.edu/technology/atlas/structures/reticulum/

Golgi Apparatus
http://www.itg.uiuc.edu/technology/atlas/structures/golgi/

Nucleus and Microtubules
http://www.itg.uiuc.edu/technology/atlas/structures/nucleus/

Nucleus and Actin
http://www.itg.uiuc.edu/technology/atlas/structures/nucleus_actin/

Membranes

Structure of Fluid Lipid Bilayers
http://blanco.biomol.uci.edu/Bilayer_Struc.html

Cell Motility

Cytoskeleton Tutorial - Basic Information (from University of Arizona Biology Project) http://www.biology.arizona.edu/cell_bio/tutorials/cytoskeleton/main.html

Cells.de - Online service for Cell Biology - Motility (Under cell Functions)
http://www.cells.de/cellseng/medienarchiv/index_medien.htm

The Borisy Lab Group
http://www.borisylab.nwu.edu/

Microscopy

Molecular Expressions Website - a little bit of everything. (Look for links to specific topics.)
http://micro.magnet.fsu.edu/

Molecular Expressions - Microscopy Primer - overview of different microscopy techniques with interactive demonstrations.
http://micro.magnet.fsu.edu/primer/virtual/virtual.html

Condenser Alignment - Koehler Illumination - Try it on-line!!
http://micro.magnet.fsu.edu/primer/java/kohler/condenseradjust/index.html

*Light Microscopy Page – contains good basic Microscopy Information
http://nsm1.fullerton.edu/%7Eskarl/EM/Microscopy/LightMicroscopy.html

*Microscopy Primer
http://www.microscopy-uk.org.uk/primer/intro.htm

Nikon Microscopy U
http://www.microscopyu.com/

Size Under the Microscope - estimating
http://www.stanford.edu/group/Urchin/size.htm

Books On-Line

Molecular Cell Biology
http://www.ncbi.nlm.nih.gov/books/bv.fcgi?call=bv.View..ShowTOC&rid=mcb.TOC

The Cell A Molecular Approach
http://www.ncbi.nlm.nih.gov/books/bv.fcgi?call=bv.View..ShowTOC&rid=cooper.TOC&depth=2

Immuno biology 5th edition
http://www.ncbi.nlm.nih.gov/books/bv.fcgi?call=bv.View..ShowTOC&rid=imm.TOC&depth=2

The NCBI Handbook
http://www.ncbi.nlm.nih.gov/books/bv.fcgi?call=bv.View..ShowTOC&rid=handbook.TOC&depth=2

History of Cell Biology

The Rockefeller University's Journey Into the Cell - includes the first electron micrograph of an intact cell
http://www.rockefeller.edu/rucal/journey/journey.html

Electron Microscopy

Boulder Laboratory for 3-Dimensional Fine Structure
http://bio3d.colorado.edu/

What Are Electron Microscopes
http://www.unl.edu/CMRAcfem/em.htm

Scanning Electron Microscopy - Includes Tutorials on SEM. Allows you to chose the level of explanation.
http://www.mse.iastate.edu/microscopy/home.html

TEM Microscopy Page
http://nsm1.fullerton.edu/~skarl/EM/Microscopy/TEMMicroscopy.html

TEM Web Course
http://em-outreach.sdsc.edu/web-course/toc.html

Bored???

Peep Research
http://www.peepresearch.org/

Bunny Survival Test
http://www.keypad.org/bunnies/index.html

The Bunnies Strike Back
http://www.marks.networktel.net/main.html

Ask Dr. Science
http://www.ducksbreath.com/

The T.W.I.N.K.I.E.S. Project
http://www.twinkiesproject.com/

Strawberry Pop-Tart Blow-Torches
http://www.pmichaud.com/toast/

Dihydrogen Monoxide Research
http://www.dhmo.org/

Science Jokes
http://www.xs4all.nl/~jcdverha/scijokes/

OXymoron Humour Archive: Scientific Articles
http://paul.merton.ox.ac.uk/science/

Whoops! Blunders and Mistakes of Science and Engineering
http://www.lhup.edu/~dsimanek/whoops.htm

A Call for More Scientific Truth in Product Warning Labels
http://paul.merton.ox.ac.uk/science/warnings.html

Minims
http://users.rcn.com/kbruhns/minims/index.html

How Much is Inside?
http://www.cockeyed.com/inside/howmuchinside.html

Encyclopedia of Days
http://www.shagtown.com/days/