Cloning

Amplification of cDNA
Competent cells
Cloning general - digests, ligations
Mini preps, dirty

Tissue Culture

Protein coIPs
FLAG-protein-RNA-coIP
Frozen stocks of tissue culture cells
Immunoflourescence microscopy
In situ hybridization
Polysome profile by sucrose gradient
Preparation of total TC RNA
Pulse-chase mRNA decay assay
siRNA transfection for HeLa and Tet-off cells
Tandem siRNA transfection
Puromycin selection

Protein work

Purification of GST-tagged protein
Affinity purification of rabbit anti-GST-tagged antigen serum
Purification of His-tagged protein
SDS-PAGE gel
Western blot

In vitro RNA work

Capping, mRNA
Decapping assay
In vitro transcription
Northern blot
Northern blot, polyacrylamide
Poly A RNA preparation
Preparation of riboprobe


Ng108 neuronal cells

Freezing Ng108s
Immunocytochemistry of Ng108s

Drosophila S2 cells

Freezing S2 cells
Fixing S2 cells onto coverslips
RNAi in S2 cells