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Lecture 14: Spaceflight Plant Chamber Design


Rationale

·        Basic Sciences: gravitropism, phototropism w/o gravity, radiation biology, gravitational biology (effect of gravity on growth, photosynthesis, materials properties, secondary metabolites, productivity, disease, genetic research,…

·        Applied Research: materials (lignin, cellulose), drugs (derived pharmaceuticals),

·        Life Support: food production, fresh food, psychological ‘green thumb’, air revitalization, water purification.

Requirements

See also: Factors affecting plant growth 

·        Light

·        Atmosphere

o       Photosynthesis (day, sufficient light, green plant shoot) / Respiration (night, germination, low light, roots): Carbon dioxide, Oxygen

o       Temperature, humidity and pressure

o       Trace contaminants

o       Air flow (mixing, heat transfer / ‘swamp cooler’), bending / shaking – affects plant morphology

·        Water and Nutrients

o       ‘root attachment’: access to water/nutrients

o       water distribution and recovery

Spaceflight Plantonauts

·        Life Support: high edible mass, fast growth, wide environmental compatibility, nutritious, flavor, low processing needs

·        Plant Biology: well characterized, environmental compatible

o       Arabidopsis – European Cress, fast growing, genome characterized, many mutants,
http://www.arabidopsis.org/, http://www.arabidopsis.com/ http://afgc.stanford.edu/

o       Wheat (life support applicability), lettuce, soy bean, pine, …..
Wisconcin Fats Plants: http://www.carolina.com/fastplants/ http://www.fastplants.org/home_flash.asp

Light:

·        Sunlight spectrum UV to IR, 1390 W/m2 extraterrestrial, 400-700 nm visible.

o       Full sunlight on earth: »2,000 umol/m2/s PAR (400-700 nm), »440W/m2,1,000 W/m2 full surface radiation), 100,000 lux (human eye only)

·        Known plant absorption blue and red (photosynthesis), UV-Blue-Red-Far-red(735nm) and ratios to each other (photomorphology)

o       Photosynthesis: energy conversion; mainly red and blue, quantum energy)

o       Photomorphogenesis: elongation (blue = short), germination (red, red-far-red ratio),

o       Photoperiod (short-day, long-day), continuous light, day/night cycle, productivity.

·        Artificial light: efficiency of electric energy to energy available to plants (leaves):

o       Lamp efficiency, distribution (point source, line source, area source) / reflectors

o       Systems engineering: operating temperature, usable spectrum: IR (heat radiation), blue content, life time bulb

·        Natural light: accessibility (safety, orbit), availability (time)

o       Clear light transmission – window / frangible

o       availability (day/night cycle LEO 40/50min of 90 min/revolution, 14day/14night moon)

o       distance to sun

Atmosphere

·        CO2 and Oxygen – consumables

o       Earth ‘typical values’: CO2: 380 ppm v/v (0.038%); 21% O2

o       Too much O2: toxicity, flammability, competitive binding of CO2

·        Moisture content – evapotranspiration – transpiration = cooling, evaporation = excessive load on humidity control / loss.

o       Typically 70% = “normal”; too high: reduced leaf evaporative cooling, mildew growth,..; too low: increased evapo-transpiration.

o       Vapor pressure – water at 25°C = 23.76 mmHg / 2.985 kPa.

·        Temperature – too low – slow growth, too high – death; plant growth very temperature-dependent; species-specific needs / optima

o       Typically 18-25°C, some plants to 30°C; most spaceflight experiments at 22-25°C

·        Pressure – low pressure plant growth possible; increased water loss, reduced heat transfer;

o       Hypobaric plant growth, low pressure plant growth,

·        Trace contaminants – harmful to plants: offgassing and biogenic production

o       Ethylene – plant hormone

·        Air flow: in microgravity w/o convection: needed for mixing, cooling, but: mechanical excitation, bending w/o gravity

o       Earth green house: recommend < 1 m/2;

o       space green house: < 0.1 m/s; vertical up / uniform distribution; no side bending (gradients, uncompensated mechanical stimuli)

Water and Nutrients:

·        Containment, control / transport / distribution in ‘soil’ w/o gravity

·        Water content sensing

·        Nutrient Delivery Systems

·        Nutrient content (salts – materials compatibility, clogging / crystallization), concentration / pH maintenance.

o       Electric conductivity (1/R = 1/Ohm = Mho = Siemens); typical nutrient solution (hydroponics: about 2 mS/cm)

o       pH (solubility changes with pH, fall-out; typically 5-6)

o       composition: macro- (N: nitrate or ammonia, P: PO4-, K” potassium) and micro-nutrients (iron, zinc,…)

o       macro (> 10 ppm): N, P, K

o       micro (< 10 ppm): Ca, Mg, Fe, Mn, B, Cu, Zn, Mo,

·        Root attachment:

o       Hydroponics, ‘soil-less’ – Rockwool, Vermiculite, Zeolite, Fibrous Ion-exchange Mats

o       Soil – pore / grain size, nutrient composition

o       Aeroponics – water mist

o       Porous tube NDS

Existing Designs  (search any payload – JSC: http://lsda.jsc.nasa.gov/scripts/cf/hw_search_result_adv.cfm )

PGU - Plant Growth Unit (single middeck locker, Kennedy Space Center)
Heyenga –gel http://quest.arc.nasa.gov/smore/events/designs/heyenga.html, Heyenga article
Chromex experiment series, PGF - Plant Growth Facility (single middeck locker,
Kennedy Space Center)

WCSAR facilities – Astroculture (1 middeck locker), Advanced Astroculture (2 middeck lockers), Commercial Plant Biotechnology Facility (CPBF – quad locker)

PGBA Plant Generic BioProcessing Apparatus (double locker, BioServe Space Technologies (MSFC Fact Sheet, ISS Fact Sheet)

BPS - Biomass Production System (double locker for ISS, Orbitec for NASA Ames / KSC) – Ames release, BPS Ames info / BRP info, MSFC fact sheet,  

SVET / LADA (Space Greenhouse, SDL Utah)

Future Designs (ISS, Mars)

Inflatable greenhouses (Mars, ISS)
”Plant(s) on Mars”
“Salad Machine” (supplemental food production for ISS, trans-mars, etc.)


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