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Katheryn RESING
Office Phone: 303 492 5519
E-mail: Katheryn.Resing@colorado.edu
FAX: 303 492 9854
Lab: Ekeley E266M
Lab Phone: 303 492 5519
Group Website:
Ph.D.: University of Washington, Seattle, 1985
Postdoctoral Fellow: University of Washington, Seattle, 1985-87
Development of High-throughput Methods for Proteomics Data Collection and Bioinformatics Analyses
My research centers around developing global protein analysis of mammalian cells. The specific technique we use involves digestion of an extract from cells into peptides, followed by multi-dimensional chromatography of the peptides, where the final stage is reverse phase coupled to a mass spectrometer. The information in this data is then used to search a protein database, in order to identify the peptides.
A large part of my research involves developing new methods for data management and mining, including new methods for validating the peptide assignments, and for quantifying and comparing different samples. We are also developing new inforamtics and computer modeling tools for utlizing this information to tackle several biological and clincial problems, that is, how does the proteome reflect modification of signaling processes in the cell, including expression, splicing, and phosphorylation.
The specific question currently under investigation are analysis of melanoma progression biology, differentiation of K562 cultured cells into erythocyte or megakaryocyte lineages, and changes in response to MKK1/2 or MKK5 in neuronal cells, specifically in hippocampus and PC12 cells.
Selected Publications
Resing, K.A., Meyer-Arendt, K., Mendoza, A.M., Aveline-Wolf, L.D., Jonscher, K.R., Pierce, K.G., Old, W.M., Cheung, H.T., Russell, S., Wattawa, J.L., Goehle, G.R., Knight, R.D., Ahn, N.G. (2004) Improving reproducibility and sensitivity in identifying human proteins by shotgun proteomics. Analytical Chemistry (revision submitted).
Bernard, K., Litman, E., Fitzpatrick, J.L., Shellman, Y.G., Argast, G., Polvinen, K., Everett, A.D., Fukasawa, K, Norris, D.A., Ahn, N.G., Resing, K.A. (2003) Functional proteomic analysis of melanoma progression. Cancer Research. 63:6716-6725.
Haydon, C.E., Eyers, P.A., Wolf, L.S., Resing, K.A., Maller, J.L. and Ahn, N.G. (2003) Identification of novel phosphorylation sites on Xenopus laevis Aurora A using improved IMAC methodology. Mol. Cell. Proteomics. 2:1055-1067.
Resing, K.A. Analysis of Signaling Pathways Using Functional Proteomics. (2002) Annals N.Y. Acad. Sci. 971:608-614.
Galasinski S., Louie D.F., Gloor, K.K., Resing, K.A., and Ahn, N.G. (2002) Global regulation of post-translational modifications on core histones. J. Biol. Chem. 277:2579-88.
Ahn, N.G. and Resing, K.A. (2001) Towards the phosphoproteome (News and Views). Nature Biotechnol. 19:317-318.
Lewis, T.S., Hunt, J.B., Aveline, L.D., Jonscher, K.R., Louie D.F., Yeh J.M., Nahreini, T.S., Resing, K.A., Ahn, N.G. (2001) Identification of Novel MAP kinase pathway signaling targets by functional proteomics and mass spectrometry. Molecular Cell. 6:1-20.
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