Biophysical Facilities

The Macromolecular X-ray Crystallographic Facility has three controlled-temperature crystallization rooms; a Phoenix robotic dropsetter for setting up crystallization arrays; two automated crystallization imaging systems (at room temperature and 4 °C); a Rigaku MM007 rotating anode with an Raxis IV++ image plate detector and cryocooling system; and a second generator and detector that will soon be upgraded.  Additionally, the facility has hardware and workstations for remote data collection at synchrotron facilities.

The Mass Spec Facility is equipped with seven mass spectrometers, including: an ABI Voyager DE-STR MALDI-TOF; an ABI 4000 Qtrap LC/MS/MS; both a Thermo LTQ-Orbitrap and a Thermo LTQ-Orbitrap Velos; a Waters Synapt G2 HDMS; an Agilent 6120 LCMS; and a Thermo Polaris Q GCMS.  The facility also possesses various liquid chromatography systems used to purify and separate sample components, including Waters nanoAcquity and Acquity UPLCs, as well as Agilent 1100 HPLCs.

The two NMR facilities on campus provide 900 MHz, 800 MHz, 600 MHz, 500 MHz (three), 400 MHz and 300 MHz spectrometers, with a wide array of probes for different nuclei and sample sizes. EPR instrumentation includes an X-band CW spectrometer equipped with standard and loop gap resonators for sample sizes as small as 10 microliters.

State-of-the-art, home-built instruments available on campus for analysis of single molecule dynamics and mechanics includes:  two single molecule TIRF fluorescence microscopy systems for monitoring fluor-tagged macromolecules on membranes or surfaces;  one single molecule fluorescence lifetime and polarization instrument for probing macromolecular dynamics;  and both laser forceps and atomic force microscopes for probing macromolecular mechanics.

The light microscopy facility includes:  a Nikon A1R laser scanning confocal and TIRF inverted microscope (7 laser lines), a Nikon N-STORM and TIRF inverted mMicroscope (4 laser lines), a Nikon Spinning Disc Confocal (7 laser lines) and a Olympus IX-81 Inverted Widefield Microscope.

Light scattering instruments available on campus include one Titan DynaPro (Wyatt Technology) dynamic light scattering (DLS) instrument for characterizing the size and mass of nanoparticles, macromolecules and complexes; two Wyatt size-exclusion multi Angle light scattering (SEC-MALS) instruments for the determination of exact molecular weight of macromolecules and complexes;and one composite-gradient multi angle laser scattering (CG-MALS) instrument to measure the binding affinities and stoichiometries of complex macromolecular interactions.

Instrumentation available on campus for thermodynamic and kinetic measurements includes two Malvern (former MicroCal) isothermal titration calorimeters; one Applied Photophysics stopped-flow spectrometer with fluorescence, absorbance and polarization monitoring capabilities; one Chirascan Plus stopped-flow spectrometer with fluorescence, absorbance, polarization, or circular dichroism monitoring; and one chemical flow-quench apparatus for quenching fast reactions at msec timepoints prior to product analysis.

Other spectroscopic and analytical instruments available on campus include four steady-state fluorescence spectrometers, two of which have fluorescence-polarization capabilities; a fluorescence-lifetime spectrometer; two circular dichroism spectrometers; femtosecond pulsed laser systems; Raman spectrometers; fluorescence cell sorters; fluorescence plate readers; some with the option of fluorescence polarization; a UV/VIS plate reader; a microplate scintillation and luminescence counter; and a Beckman analytical ultracentrifuge.