research

April Kloxin and Katie Lewis publish "Responsive Culture Platform to Examine the Influence of Microenvironmental Geometry on Cell Function in 3D" in Integrative Biology

November 6, 2012

April, now an assistant professor at University of Delaware, and Katie presented a dynamic culture platform in which microwells are patterned and later connected through cytocompatible photocleavage of a poly(ethylene glycol)-based hydrogel to examine matrix geometry effects on cell fate.

Chelsea Kirschner publishes "In Situ Control of Cell Substrate Microtopographies Using Photolabile Hydrogels" in Small

November 6, 2012

Chelsea demonstrated that human Mesenchymal Stem Cells align along topographies patterned on the surface of photodegradable hydrogels. She further showed that cells will dynamically change their cytoskeletal organization in response to a modified topography.

Cole DeForest publishes "Bioactive Hydrogels for Regenerative Medicine" in Annual Review of Chemical and Biomolecular Engineering

October 18, 2012

Former group member Cole DeForest, now performing postdoctoral research under David Tirrell, published an excellent review of cutting edge bioactive hydrogel reserach titled "Bioactive Hydrogels for Regenerative Medicine" in Annual Review of Chemical and Biomolecular Engineering.

Abby Bernard publishes "A Microwell Culture Platform for the Aggregation of Pancreatic Beta-Cells" in Tissue Engineering Part C-Methods

September 4, 2012

Abby Bernard showed that pancreatic beta-cells aggregates could be formed in photopolymerized microwell devices. These cell aggregates secreted significantly more insulin per cell than dispersed cells.

A.B. Bernard, C.C. Lin, and K.S. Anseth, "A Microwell Culture Platform for the Aggregation of Pancreatic Beta-Cells," Tissue Engineering Part C-Methods, 18 (8), 583-592 (2012).

Josh McCall publishes "Thiol-Ene Photopolymerizations Provide a Facile Method to Encapsulate Proteins and Maintain Their Bioactivity" in Biomacromolecules

August 30, 2012

Recent Anseth Group Alum Josh McCall showed the that proteins encapsulated in hydrogels formed by radical-mediated thiol-ene polymerization maintain significantly more activity than those encapsulated in gels formed by more traditional acrylate polymerization.

J.D. McCall and K.S. Anseth, "Thiol-Ene Photopolymerizations Provide a Facile Method to Encapsulate Proteins and Maintain their Bioactivity," Biomacromolecules, 13 (8), 2410-2417 (2012).

Sarah Gould publishes “Small Peptide Functionalized Thiol-Ene Hydrogels as Culture Substrates for Understanding Valvular Interstitial Cell Activation and de novo Tissue Deposition” in Acta Biomaterialia

August 22, 2012

Sarah Gould showed VICs significantly upregulate alpha-SMA when plated on gels functionalized elastin and collagen mimic peptides as compared with the canonical fibronectin mimic peptide RGD. Furthermore, Sarah showed that alpha-SMA expression, indicative of a disease phenotype, is downregulated in VICs plated on gels versus though on tissue culture plastic.

Malar Azagarsamy publishes "Photocontrolled Nanoparticles for On-Demand Release of Proteins" in Biomacromolecules

August 21, 2012

Malar Azagarsamy demonstrated the light-mediated release of protein from PEG nanogels. He showed the release of active ALP in vitro and that nanogels enter the cell when coupled to TAT peptides.

M.A. Azagarsamy, D.L. Alge, S.J. Radhakrishnan, M.W. Tibbitt, and K.S Anseth, "Photocontrolled Nanoparticles for On-Demand Release of Proteins," Biomacromolecules, 13 (8), 2219-2224 (2012).

Sharon Wang publishes "Redirecting Valvular Myofibroblasts into Dormant Fibroblasts through Light-Mediated Reduction in Substrate Modulus" in PLoS ONE

August 20, 2012

Sharon (Huan) Wang shows that valvular interstitial cells will return to a quiescent phenotype after reducing the modulus of the substrate on which they are plated using the nitrobenzyl photochemistry the Anseth group brought into hydrogels. The figure shows the reduction in alpha smooth muscule actin (green) in gels that have been softened.

Mark Tibbit publishes "Synthesis and Application of Photodegradable Microspheres for Spatiotemporal Control of Protein Delivery" in Journal of Biomedical Materials Research"

August 20, 2012

Mark Tibbitt demonstrated controlled release of protein from photodegradable PEG microspheres. His work earned him the Outstanding Research Winner in the PhD category for the 9th World Biomaterials Congress in China.

M.W. Tibbitt, B.W. Han, A.M. Kloxin, and K.S. Anseth, "Synthesis and Application of Photodegradable Microspheres for Spatiotemporal Control of Protein Delivery," Journal of Biomedical Materials Research Part A, 100A (7), 1647-1654 (2012).

Pat Hume publishes "Strategies to Reduce Dendritic Cell Activation through Functional Biomaterial Design" in Biomaterials

August 20, 2012

Pat Hume showed that immunosuppressive factors maintain their activity when tethered to a hydrogel and reduce markers of dendritic cell activation. The figure shows reduced levels of IL-12 expression in dendritic cells seeded upon hydrogels functionalized with immunosuppressive proteins TGF-B1 and IL-10.

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