I am developing a dynamic hydrogel cell culture platform for studying heart disease. Using molecular biology and engineering approaches, I seek to analyze biological systems on multiple length and time scales. I am culturing neonatal rat ventricular myocytes (NRVMs) and fibroblasts (NRVFs) on poly(ethylene glycol) hydrogel systems to mimic diseased and healthy cellular microenvironments. These gel systems will have the capability for non-invasive, sequential increases and decreases of substrate modulus. These studies will help engineers and molecular biologists improve designs of engineered heart tissue and increase the fundamental understanding of the progression and regression of heart disease.
Wan, W., & Gleason, R. L. Dysfunction in elastic fiber formation in fibulin-5 null mice abrogates the evolution in mechanical response of carotid arteries during maturation. American journal of physiology. Heart and circulatory physiology, 304(5), H674–686, 2012.
Wan W, Dixon JB, Gleason RL. Constitutive Modeling of Mouse Carotid Arteries Using Experimentally Measured Microstructural Parameters. Biophysical Journal, 102(12), 2916-2925, 2012.
Wan W, Hansen L, and Gleason RL. A 3-D constrained mixture model for mechanically mediated vascular growth and remodeling. Biomechanics and Modeling in Mechanobiology 9: 403-419, 2010.
Wan W, Yanagisawa H, and Gleason RL. Biomechanical and Microstructural Properties of Common Carotid Arteries from Fibulin-5 Null Mice. Annals of Biomedical Engineering 38: 1-13, 2010.