Human mesenchymal stem cells (hMSC) are pluripotent, adult stem cells that are recruited from the stem cell niche to bone fracture sites to colonize the defect, proliferate, release trophic cues for therapeutic regulation and differentiate. A more complete understanding of the mechanisms that are essential to MSC migration and recruitment would enable the design of materials to accelerate bone regeneration. To this end, our group is investigating ways to develop cell constructs that can be used to facilitate cell infiltration or used as a cell carrier platform. In this work, we employed thiol-ene based hydrogels that can be functionalized with relevant proteins and small peptide mimics to recapitulate important mechanical and biological cues that a cell would receive from their in vivo extracellular microenvironment. The fundamental understanding of MSC migration garnered from these materials will lead to better engineering of polymeric materials that facilitate the recruitment of endogenous mesenchymal stem cells to bone defect sites.