CORE FACILITIES AVAILABLE TO SCR PARTICIPANTS

Biomolecular mass spectrometry:       A research, training, and service facility is housed in the Ekeley Building includes a PerSeptive Voyager DE-STR MALDI-TOF mass spectrometer, an ABI QStar Pulsar qTOF mass spectrometer with interchangeable ESI and oMALDI interfaces, three electrospray ion trap mass spectrometers (Finnigan LCQ Classic, Finnigan Deca XP, Agilent trap). The four qTOF and ion trap instruments are each outfitted with an Agilent Model 1100 capillary HPLC with autosampler. Instrument maintenance and student training are carried out by Drs. Natalie Ahn and William Old. These instruments are housed in the Central Analytical Facility, which also maintains a VG AutoSpec triple sector EBE machine, with chemical ionization, electron impact, liquid SIMS and ESI sources, an HP 5989A electrospray ionization single quadrupole mass spectrometer, and an HP5988A gas chromatography mass spectrometer. The latter instruments are operated by Dr. Shuji Kato, staff specialist in the Central Analytical Facility, and are used mainly for organic and analytical samples.

Transgenic Facility:           The facility was constructed with support from the William Keck Foundation. The primary mission of this facility is to provide a barrier environment for the housing of valuable strains of mice and a core facility for use in the generation of transgenic mice. These mouse strains are generated to study problems in basic biology and disease. Investigators using the facility are presently working with strains of mice that have the potential to lead to new insights into heart disease, neurodegenerative disease, and diabetes. The facility is managed by Drs. Kevin Jones and Gail Ackermann.

NMR: A facility containing 500 and 600 MHz 4-channel Varian Inova NMR spectrometers and a three channel Varian Inova 500 MHz spectrometer is located in the Cristol Chemistry Building and maintained and operated by Drs. Arthur Pardi and Deborah Wuttke, with staff specialist Richard Shoemaker. A second facility (the W. M. Keck 800 MHz NMR Facility) is located off campus in Boulder and houses a Varian 800 MHz NMR with cryogenic probe. This facility is a consortium between three institutions: CU-Boulder, CU-Denver and the University of Utah. A third facility (the Rocky Mountain Regional 900 MHz NMR Facility) is located at the Anschutz Medical campus and houses a Varian 900 MHz NMR to serve the high field NMR requirements of publicly funded research institutions in Rocky Mountain/West Central states. The consortium consists of 17 research groups from 9 institutions in 6 states. Maintenance and training of users are carried out by Dr. Geoff Armstrong.

Biomolecular X-ray crystallography:   A facility in the Cristol Building houses two X-ray generators equipped with cryosystems and image-plate detectors; a microfluidics crystallizer, a nanodrop setter for crystallizations, and an automated imager for crystallization analysis. The X-ray facility is maintained by Drs. David McKay, Marcelo Sousa, Robert Batey, and Tom Cech. Dr. McKay trains students in the use of the instruments and analysis of data.

National Research Resource laboratory for three-dimensional electron microscopy:         The facility is located in Porter Biosciences and currently supported by a grant from the NIH National Center for Research Resources under the directorship of Dr. Andreas Hoenger. In this lab, methods have been developed for EM tomography of cells, allowing 3-dimensional reconstruction of cells with resolutions of ~2 nm. Methods have also been developed for serial section and immunolabeling at the EM level to localize specific antigens in 3-D within the context of cellular structure. This Resource operates a JEOL 1000 high voltage electron microscope, equipped with a goniometer stage for ambient temperature work and a single axis tilting cold stage. The Facility also has 200 and a 300 KeV instruments from FEI, Inc. Each is equipped with field emission gun, goniometer stage, and CCD camera. The 300 KeV is also equipped with a Gatan Imaging Filter to permit acquisition of images based on energy filtered electrons. Supporting computer facilities for tomographic reconstruction and serial section image processing are also available.

Fluorescence microscopy:         Chemistry and Biochemistry, MCDB and CBEN have extensive facilities for microscopic analysis of cellular structure. An electron microscopy suite equipped with two Philips CM10 100 KeV electron microscopes are housed in MCDB, together with a Balzers high pressure rapid freezer and ultrahigh vacuum-ultralow temperature freeze fracture apparatus. The department also operates facilities for 3D reconstruction, with a Molecular Dynamics scanning confocal microscope. A Zeiss LSM5 Pascal scanning confocal microscope is housed in the CBEN building. Three upright deconvoluting light microscopes are housed in the labs of Han, McIntosh, and Winey. Zeiss Axioplan upright and Leica inverted fluorescence microscopes with digital CCD cameras are located in the Cristol Building and shared by Chemistry and Biochemistry laboratories.

Single molecule fluorescence and FRET:       A Keck Foundation supported facility that contains a CCD-based Total Internal Reflection Fluorescence (TIRF) microscope is housed in Cristol Chemistry. The instrument consists of an objective-based total internal reflection fluorescence microscope (Nikon TE-2000U) equipped with a Cascade II CCD camera and a 1.49 NA water immersion objective. The facility is maintained by Dr. Arthur Pardi.

Fluorescence spectroscopy:     A SLM 48000S fluorescence spectrometer equipped for steady state and phase modulation lifetime measurements, with laser (Coherent Innova 306 argon ion laser) and arc lamp (Xe or Hg-Xe) excitation sources, is located in the Cristol Building and maintained by Dr. Joe Falke.

DNA sequencing:   A service facility located in the Porter Biosciences Building is equipped with two ABI Prism 377 automated DNA sequencers, and is operated by Dr. Yuming Han and two work study students.

DNA microarray:   A facility to process and read Affymetrix chips is housed in the MCDB addition and operated by Ms. Helen Marshall, under the supervision of Drs. Ken Krauter and Ryan Gill. The facility includes an Agilent G2500A GeneArray scanner with fluidics station, hybridization oven, computer workstation, and an Agilent 2100 bioanalyzer.

Computer cluster: A 200-core Linux cluster is located in the Porter Biosciences building and maintained by Dr. Rob Knight.

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