2010 SCR Symposium

Monday, May 10, 2010   ATLAS Bldg, Room 100

8:45-9:30 am Breakfast, ATLAS Lobby


9:30 am-12:00 pm  Morning Session, Room 100

          Kim Orth, U Texas Southwestern  “Black spot, black death, black pearl – The tales of bacterial effectors”

          Coffee Break

10:40 am-12:00 pm  Morning Session continues

           Douglas Chapnick “TGFb regulates cell motility and cell-cell junctions through the protein tyrosine phosphatase PTP-PEST”

           Marina Kniazeva "Biological function of cellular lipids in C. elegans; branching from usual”

           Philip Dittmer "Tracking cellular Zn- reveals a novel connection between Ca and Zn in the ER”

           Mara Stewart "An in vivo screen for radiation sensitizers"


12:00-1:15 pm  Lunch, ATLAS lobby


1:15-3:30 pm Afternoon Session, Room 100

            Carrie Bernecky "Molecular architecture of the human Mediator-Pol II-TFIIF assembly"

            Nathan Gomes "Differential regulation of p53 target gene expression via intragenic CTCF-Cohesin binding"

            Deanne Sammond "Deciphering the signaling mechanism of a viral oncoprotein: Studying the EBV protein LMP1"

            Jesse Zaneveld "Exploring the evolution of genome content in gut-adapted bacteria"

            Anne Brunet, Stanford Univ. "Mechanisms of aging & longevity"


Plenary Speakers

           Dr. Kim Orth is Associate Professor of Molecular Biology at University of Texas, Southwestern.  Dr. Orth is a leader in understanding virulence factors from pathogenic bacteria.  These include Type III secretion system effectors, which have evolved in a manner similar to many of the viral oncogenes, in which a eukaryotic activity is usurped and modified by the pathogen for its own advantage.  Dr. Orth is known for her discovery that the virulence gene Yop J from Yersinia pestis -- the bacteria responsible for bubonic plague – works by catalyzing a novel enzymatic reaction involving Ser/Thr acetylation, thus blocking residues in MAPKKs which are normally phosphorylated and disrupting innate immune responses.  She also discovered that the VopS gene from Vibrio parahaemolyticus works by AMPylation of RhoGTPases at Thr residues which disrupt effector binding.  Dr. Orth’s work has led to new insight into how virulence factors disrupt cell signaling mechanisms, and at the same time revealed protein post-translational modifications that have never before been described.      http://www4.utsouthwestern.edu/orthlab/


   Broberg, C.A., & Orth, K. Tipping the balance by manipulating post-translational modifications. (2010) Curr Op Microbiol. (Epub 2010 Jan 11).  

   Mukherjee, S. Kietany, G., Li, Y Wang, Y., Hall B.E., Goldsmith, E & Orth, K. Yersinia YopJ Acetylates and Inhibits Kinase Activation by Preventing Phosphorylation. (2006) Science, 312:1211-1214.

   Yarbrough, M., Li, Y., Kinch, L.N., Grishin, N.V., Hall B.E., & Orth, K. AMPylation of Rho GTPases by Vibrio VopS disrupts effector binding and downstream signaling. (2009) Science. 323: 269-272.


           Dr. Anne Brunet is Associate Professor of Genetics at Stanford Univ.  Her laboratory studies the molecular mechanisms of aging and longevity, with particular interests in the aging of the nervous system.  Their work focuses on 'longevity genes' such as FOXO family of Forkhead transcription factors and Sirtuins, and how these genes control gene expression and chromatin status in response to environmental stimuli, including nutrient deprivation and DNA damage.  Dr. Brunet is known for the discovery that in mammalian cells, the Sir2 homolog SIRT1 controls cellular stress responses by regulating FOXO transcription factors, through complex formation and SIRT1 deacetylation of FOXO.  As a result, SIRT1 increases FOXO's ability to induce cell cycle arrest and resistance to oxidative stress but inhibits FOXO's ability to induce cell death. Thus, Sirtuins may increase longevity by tipping FOXO-dependent responses away from apoptosis and toward stress resistance.  Her lab is investigating these mechanisms in neural stem cells, C. elegans, and African killfish, to understand their impact on learning and memory during aging and their link with dietary restriction.           http://www.stanford.edu/group/brunet/


   Brunet A. Cancer: when restriction is good. (2009) Nature. 458: 713-714.

   Greer EL and Brunet A. Different dietary restriction regimens extend lifespan by both independent and overlapping genetic pathways in C. elegans. (2009) Aging Cell. 8: 113-127.

   Brunet A, et al. Stress-dependent regulation of FOXO transcription factors by the SIRT1 deacetylase. (2004) Science 303: 2011-2015.

   Renault VM, et al.  FoxO3 regulates neural stem cell homeostasis.  Cell Stem Cell. 2009 Nov 6;5(5):527-39



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