Protocols of the Odorizzi Lab
Molecular Biology
Preparation of Yeast Genomic DNA for PCR
TOPO-cloning PCR products
Transformation of Bacteria
Plasmid DNA miniprep by alkaline lysis
Construction of Genomic DNA Library from Yeast
Preparation of Competent BL21 Bacteria
Preparation of Competent Bacteria (Inoue Method)
Pulldown of GST proteins mixed with bacterial lysates
Preparation of 5X Orange G Loading Dye and 1-kb ladder
Yeast Methods
Transformation of Yeast
High-Efficiency Transformation of Yeast with Plasmid Library DNA
Lysis of Whole Yeast Cells for SDS-PAGE
Preparation of Yeast Spheroplasts
Protease Inhibitors
FM 4-64 Staining of Yeast Vacuoles
Indirect Immunofluorescence (Courtesy of Markus Babst, Univ of Utah)
Western Blot Analysis of Ubiquitin (Courtesy of Randy Hampton, UCSD)
Equipment
useful links
Lab Calendar: HERE
people in our lab
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Our group consists of postdocs, graduate students, technicians, and undergraduate students. If you are interested in joining us, email the PI, Greg Odorizzi.