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Molecular Biology Protocols
PCR
PCR from Yeast Genomic DNA Template
Preparation of Yeast Genomic DNA for PCR
TOPO-cloning PCR products Restriction
Digestion of Plasmid DNA
Transformation of Bacteria
Plasmid DNA miniprep
Construction of Genomic
DNA Library from Yeast
Yeast Methods
Transformation of Yeast
Lysis of Whole Yeast Cells for SDS-PAGE
Preparation of Yeast Spheroplasts
Protease Inhibitors
FM 4-64 Staining of Yeast Vacuoles
Indirect Immunofluorescence
(Courtesy of Markus Babst, Univ of Utah)
Western Blot Analysis of Ubiquitin
(Courtesy of Randy Hampton, UCSD)