MCDB 1151, Fall 2007 SCHEDULE OF LABORATORIES

Week of           

Title    

Reading

Independent Project Schedule

Aug. 27

Introduction

Sept. 3

References, Scientific Papers, Liquid Measurements

pp. 10-34, 62

Protein

Sept. 10

Proteins:  Assaying enzyme activity from recombinant cells

Pp 64-70

Choose experimental question

Energy Transduction and Cells

Sept. 17

ATP-Biological Currency

pp. 72-79

Design experiment

Sept. 24

Cells

pp. 81-89

pp. 51-57

Set up reagents

Oct. 1

Independent Projects

pp. 59-60

pp. 139-140

Conduct experiment

DNA

Oct. 8

DNA Replication and PCR, Isolating DNA from genetically modified organisms

pp. 91-98

Oct. 15

Electrophoresis of PCR products

pp. 98-100

First Lab Report Due

Oct. 22

Recombinant DNA, Competent Cell preparation, Transformation

pp. 102-116

Pick experimental question for second independent project

Oct. 29

Recombinant DNA, Mini Prep and Restriction Digest

pp. 116-121

Design experiment

Nov. 5

Gel electrophoresis of mini prep restriction digests

pp. 121-127

Set up reagents

Nov. 12

Independent Projects, DNA

pp.  59-60 pp. 139-140

Conduct Experiment

Nov. 19

Thanksgiving Holiday, no labs

Nov. 26

Independent Projects, DNA cont.

Complete experiment

Dec. 3

Power Point Session

Second Lab Report due

Dec. 10

Power Point Session continued, end of semester review