Cecilia A. Riquelme, Ph.D.
Home Address:
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Work Address: |
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2300 Arapahoe St. Apt. 105
Boulder,
CO 80302 Home
Phone: (303) 786-1063 Email:
Cecilia.Riquelme@colorado.edu |
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University
of Colorado at Boulder Chemistry
and Biochemistry Department, Campus Box 215 Boulder,
CO 80309 |
Lab
Phone:(303) 492-3804 Fax:
(303) 735-6161 |
Personal
Data:
Education:
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Pontificia
Universidad Católica de Chile, Santiago |
Ph.D. |
2001 |
Cellular & Molecular Biology |
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Universidad de Concepcion, Concepcion |
B.S. |
1996 |
Biochemistry |
Research
Experience:
Postdoctoral Work:
“Posttranslational modifications by SUMO
and muscle differentiation”. Advisor: Dr. Xuedong Liu.
Doctoral Thesis:
“Regulation of muscle differentiation by decorin: control
of the TGF-b binding to its receptors”. Advisor: Dr. Enrique Brandan,
Pontificia Universidad Católica de Chile, Santiago.
M.S. Thesis:
“House dust mite allergens: immunological and structural
study”. Mentor: Dr. Jose Martinez, Universidad de Concepcion, Concepcion.
Positions Held
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University of
Colorado at Boulder |
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Department of Chemistry and Biochemistry |
Postdoctoral (Research Fellow) |
2002-Present |
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Pontificia Universidad Católica de Chile |
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Department of Cellular and Molecular Biology |
Associate Investigator |
2001-2002 |
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Teaching Assistant, Cell biology Laboratories |
1998-1999 |
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Publications:
Riquelme C, Larrain J, Schonherr E,
Henriquez JP, Kresse H, Brandan E. (2001). Antisense
inhibition of decorin expression in myoblasts decreases cell responsiveness to transforming
growth factor beta and accelerates skeletal muscle differentiation. J Biol Chem 276(5):
3589-96
Lopez-Casillas F, Riquelme C, Perez-Kato Y, Ponce-Castaneda
MV, Osses N, Esparza-Lopez J, Gonzalez-Nunez G, Cabello-Verrugio C, Mendoza V,
Troncoso V, Brandan E. (2003). Betaglycan
expression is transcriptionally up-regulated during skeletal muscle
differentiation. Cloning of murine betaglycan gene promoter
and its modulation by MyoD, retinoic acid, and transforming growth factor-beta.
J Biol Chem 278(1): 382-90
Cheung HT, Collins PJ, Riquelme C,
Kwan P, Shannon K and Liu X. (2004) Specificity of TGF-beta and Activin A
signaling responses revealed by the analysis of their transcriptional programs
(submitted to Mol Cell Biol).
Cabello-Verrugio C, Riquelme C
and Brandan E. (2004) Augmented binding of TGF-b to betaglycan in
decorin-null myoblasts. J Cell Phys (revised form).
Riquelme C, Barthel K and Liu X. (2004) Sumo-1 modification of MEF2A
regulates its transcriptional activity (submitted to JBC).
Riquelme, C, Barthel, KKB and Liu, X. “Ubc9 is essential for muscle
differentiation”. Annual
Meeting of the Biochemistry Department of the University of Colorado, Winter
Park (USA). September 9th-10th, 2004 (oral
communication).
Brandan E, Cabello-Verrugio, C
and Riquelme, C. Gordon Conference,
Il Ciocco (2004).
Riquelme, C and Brandan E. “Decorin as a repressor of skeletal muscle
differentiation by TGF-b dependent mechanism”. 40th American Society for Cell Biology,
Annual Meeting, San Francisco (USA); December 9th-12th,
2000 (poster session).
Riquelme, C and Brandan E. “Decorin antisense myoblasts have a
constitutive expression of myogenin and they differentiate faster than wild
type myoblasts”. ASILOMAR, San Francisco (USA); May 22th-26th,
2000 (poster session).
Riquelme, C and Brandan E. “Decorin modulates skeletal muscle
differentiation”. 39th
American Society for Cell Biology, Annual Meeting, Washington DC (USA);
December 11th-15th, 1999 (poster session).
Riquelme, C. “Inhibition of decorin expression increases both muscular
differentiation and TGF-b2 binding to betaglycan”. XIII Annual
Meeting of the Chilean Society for Cell Biology, Pucon (Chile). November
16th-20th, 1999 (oral communication).
Riquelme, C. “Decorin: an extracellular matrix proteoglycan that could
modulate skeletal muscle differentiation”. XII Annual Meeting
of the Chilean Society for Cell Biology, Valdivia (Chile). September 22th-25th,
1998 (oral communication).
Riquelme, C. “Possible interaction between decorin and TGF-b
during myogenesis”. XL
Annual Meeting of the Chilean Society for Biology, Pucon (Chile).
November 22th - 25th, 1997 (oral communication).
Fellowships,
Honors and Awards:
Graduated Magna Cum Laude, Pontificia Universidad Católica
de Chile – 2001
National Committee on Science and Technology
(CONICYT-Chile) Graduate Student Research Fellowship to Complete PhD Thesis,
2000
National Committee on Science and Technology
(CONICYT-Chile) Graduate Student Research Fellowship, 1996-1999
Scientific Societies:
Member of the Chilean Society for Cell Biology, 2001
Skills:
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DNA: molecular cloning, minipreps, maxipreps, PCR, gel
shifts. RNA: quantitative RT-PCR, northern blots. Proteins: SDS-PAGE, ND-PAGE, kinase assays, general
chromato-graphy techniques, radioactive labeling of cells (3H, 35S,
32P), pulse-chase, radioactive-ligand
binding, iodination, bacterial expression, GST-purification, GST-pulldown. Immunological
techniques: affinity purification of
antibodies, immunoprecipitation, western blotting, immunofluorescence,
immuno-histochemistry, ELISA., fluorescent-activated cell sorting (FACS) Cell Culture: primary and cell lines, transient and stable
transfection, adenoviral and retroviral infections. |
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Grants:
Principal Investigator, “Decorin regulates the biological
activity of TGF-b: effect on skeletal muscle differentiation”. National Fund
for Science and Technology Development (FONDECYT, Chile), Graduate Student
Section # 2980053, 1998-2000.
Principal Investigator, “Regulation of skeletal muscle
differentiation by decorin: effect on TGF-b binding to its receptors”. National Fund for Science and Technology Development (FONDECYT,
Chile), Graduate Student Section # 4000005, 2001.